Since the beginning of 90s, the advent of mammalian gene engineering technology brought the mouse into a central role for investigations of the mammalian central nervous systems. This led to our renewed interest in mouse brain anatomy. As anatomical guidance, mouse brain atlases have been a crucial resource in various aspects of neuroscience studies. These include anatomical references to detect abnormalities, identification of coordinates of specific structures for stereotaxic operations, and templates for registering and reporting locations of histology-based results. The overall goal of this project is to develop and provide MRI-based atlases of developing mouse brains. Most of the currently available atlases are based on digitized histology slices. While they can provide detailed views of brain anatomy, the accuracy of the stereotaxic coordinates could be compromised by distortions related to fixation and sectioning processes. The slice orientations and locations are limited and for stereotaxic operations, the 2D nature of the histology-based atlases is often a severe limitation. In the past grant period, we enhanced our diffusion tensor microimaging technology (Aim 2), established normal brain development database (Aim 1), performed histology-MRI comparison (Aim 3), and developed quantitative analysis tools (Aim 4). We will use these database, technology, and experiences as core resources to propose the renewal of this grant. For the next 5 years, we set the following three aims;
Aim 1 : To establish atlases of developing mouse brains with full segmentation and annotation Aim 2: To develop image-guided stereotaxic operation of developing mouse brains Aim 3: To provide tools for atlas-based automated anatomical analyses for abnormal brain growth Aim 4: To disseminate the atlases, image-registration tools, and use interface software

Public Health Relevance

In this project, we will develop brain atlases of developing mouse brains. Unlike existing histology- based 2D atlases, our atlases will be based on three-dimensional MRI data. The atlases will provide greater accuracy for stereotaxic operations such as delivery of drags to a specific coordinates in the brain. The atlas will also allows us to perform automated segmentation of the brain after MRI studies of various types of mouse disease models. We believe that these atlases will be important resources for a wide range of neuroscience researches.

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Research Project (R01)
Project #
5R01EB003543-08
Application #
8291908
Study Section
Medical Imaging Study Section (MEDI)
Program Officer
Pai, Vinay Manjunath
Project Start
2004-04-01
Project End
2014-07-31
Budget Start
2012-08-01
Budget End
2014-07-31
Support Year
8
Fiscal Year
2012
Total Cost
$647,075
Indirect Cost
$201,607
Name
Johns Hopkins University
Department
Radiation-Diagnostic/Oncology
Type
Schools of Medicine
DUNS #
001910777
City
Baltimore
State
MD
Country
United States
Zip Code
21218
Dada, Tahani; Rosenzweig, Jason M; Al Shammary, Mofeedah et al. (2014) Mouse model of intrauterine inflammation: sex-specific differences in long-term neurologic and immune sequelae. Brain Behav Immun 38:142-50
Wu, Dan; Reisinger, Dominik; Xu, Jiadi et al. (2014) Localized diffusion magnetic resonance micro-imaging of the live mouse brain. Neuroimage 91:12-20
Carver, Alissa R; Andrikopoulou, Maria; Lei, Jun et al. (2014) Maternal pravastatin prevents altered fetal brain development in a preeclamptic CD-1 mouse model. PLoS One 9:e100873
Aggarwal, Manisha; Burnsed, Jennifer; Martin, Lee J et al. (2014) Imaging neurodegeneration in the mouse hippocampus after neonatal hypoxia-ischemia using oscillating gradient diffusion MRI. Magn Reson Med 72:829-40
Wu, Dan; Xu, Jiadi; McMahon, Michael T et al. (2013) In vivo high-resolution diffusion tensor imaging of the mouse brain. Neuroimage 83:18-26
Aggarwal, Manisha; Zhang, Jiangyang; Pletnikova, Olga et al. (2013) Feasibility of creating a high-resolution 3D diffusion tensor imaging based atlas of the human brainstem: a case study at 11.7 T. Neuroimage 74:117-27
Ratnanather, J Tilak; Lal, Rakesh M; An, Michael et al. (2013) Cortico-cortical, cortico-striatal, and cortico-thalamic white matter fiber tracts generated in the macaque brain via dynamic programming. Brain Connect 3:475-90
Li, Xin; Aggarwal, Manisha; Hsu, Johnny et al. (2013) AtlasGuide: software for stereotaxic guidance using 3D CT/MRI hybrid atlases of developing mouse brains. J Neurosci Methods 220:75-84
Aggarwal, Manisha; Duan, Wenzhen; Hou, Zhipeng et al. (2012) Spatiotemporal mapping of brain atrophy in mouse models of Huntington's disease using longitudinal in vivo magnetic resonance imaging. Neuroimage 60:2086-95
Jiang, Mali; Wang, Jiawei; Fu, Jinrong et al. (2012) Neuroprotective role of Sirt1 in mammalian models of Huntington's disease through activation of multiple Sirt1 targets. Nat Med 18:153-8

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