The goal of this Bioengineering Research Partnership application is pursue the next phase of development and application of Raman flow cytometry. In the initial project period, we designed and built the first Raman flow cytometers and demonstrated that they have the analysis speed and sensitivity for a wide range of applications. In the second project period, we will develop a Raman-enhanced flow cytometer with both Raman and fluorescence measurement capabilities, develop a new generation of uniform SERS nanotags, and use these in multiparameter analysis of cell signaling pathways. The result of this work will be a significant new tool for the highly multiparameter analysis of cell systems to help understand, diagnose, and prevent disease.
Project Narrative This project aims to significantly increase the analysis capabilities of flow cytometry by incorporating Raman spectral analysis capabilities. The result of this work will be a significant new tool for the highly multiparameter analysis of cell systems to help understand, diagnose, and prevent disease.
|Stoner, Samuel A; Duggan, Erika; Condello, Danilo et al. (2016) High sensitivity flow cytometry of membrane vesicles. Cytometry A 89:196-206|
|Mulligan, SÄ—an K; Speir, Jeffrey A; Razinkov, Ivan et al. (2015) Multiplexed TEM Specimen Preparation and Analysis of Plasmonic Nanoparticles. Microsc Microanal 21:1017-25|
|Nolan, John P; Duggan, Erika; Condello, Danilo (2014) Optimization of SERS tag intensity, binding footprint, and emittance. Bioconjug Chem 25:1233-42|
|Zhu, Shaobin; Ma, Ling; Wang, Shuo et al. (2014) Light-scattering detection below the level of single fluorescent molecules for high-resolution characterization of functional nanoparticles. ACS Nano 8:10998-1006|
|Nolan, John P; Stoner, Samuel A (2013) A trigger channel threshold artifact in nanoparticle analysis. Cytometry A 83:301-5|
|Nolan, John P; Condello, Danilo (2013) Spectral flow cytometry. Curr Protoc Cytom Chapter 1:Unit1.27|
|Nolan, John P; Condello, Danilo; Duggan, Erika et al. (2013) Visible and near infrared fluorescence spectral flow cytometry. Cytometry A 83:253-64|
|Orjuela-Sanchez, Pamela; Duggan, Erika; Nolan, John et al. (2012) A lactate dehydrogenase ELISA-based assay for the in vitro determination of Plasmodium berghei sensitivity to anti-malarial drugs. Malar J 11:366|
|Nolan, John P; Duggan, Erika; Liu, Er et al. (2012) Single cell analysis using surface enhanced Raman scattering (SERS) tags. Methods 57:272-9|
|Nolan, John P; Sebba, David S (2011) Surface-enhanced Raman scattering (SERS) cytometry. Methods Cell Biol 102:515-32|
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