The goal of this Bioengineering Research Partnership application is pursue the next phase of development and application of Raman flow cytometry. In the initial project period, we designed and built the first Raman flow cytometers and demonstrated that they have the analysis speed and sensitivity for a wide range of applications. In the second project period, we will develop a Raman-enhanced flow cytometer with both Raman and fluorescence measurement capabilities, develop a new generation of uniform SERS nanotags, and use these in multiparameter analysis of cell signaling pathways. The result of this work will be a significant new tool for the highly multiparameter analysis of cell systems to help understand, diagnose, and prevent disease.

Public Health Relevance

This project aims to significantly increase the analysis capabilities of flow cytometry by incorporating Raman spectral analysis capabilities. The result of this work will be a significant new tool for the highly multiparameter analysis of cell systems to help understand, diagnose, and prevent disease.

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Research Project (R01)
Project #
7R01EB003824-11
Application #
8878748
Study Section
Special Emphasis Panel (ZRG1-SBIB-V (50))
Program Officer
Lash, Tiffani Bailey
Project Start
2004-09-01
Project End
2015-02-28
Budget Start
2014-09-15
Budget End
2015-02-28
Support Year
11
Fiscal Year
2014
Total Cost
$663,399
Indirect Cost
$281,786
Name
Scintillon Institute for Photobiology
Department
Type
DUNS #
078367362
City
San Diego
State
CA
Country
United States
Zip Code
92121
Zhu, Shaobin; Ma, Ling; Wang, Shuo et al. (2014) Light-scattering detection below the level of single fluorescent molecules for high-resolution characterization of functional nanoparticles. ACS Nano 8:10998-1006
Nolan, John P; Duggan, Erika; Condello, Danilo (2014) Optimization of SERS tag intensity, binding footprint, and emittance. Bioconjug Chem 25:1233-42
Nolan, John P; Condello, Danilo; Duggan, Erika et al. (2013) Visible and near infrared fluorescence spectral flow cytometry. Cytometry A 83:253-64
Nolan, John P; Condello, Danilo (2013) Spectral flow cytometry. Curr Protoc Cytom Chapter 1:Unit1.27
Nolan, John P; Stoner, Samuel A (2013) A trigger channel threshold artifact in nanoparticle analysis. Cytometry A 83:301-5
Nolan, John P; Sebba, David S (2011) Surface-enhanced Raman scattering (SERS) cytometry. Methods Cell Biol 102:515-32
Chhabra, Rahul; Moralez, Jesus G; Raez, Jose et al. (2010) One-pot nucleation, growth, morphogenesis, and passivation of 1.4 nm Au nanoparticles on self-assembled rosette nanotubes. J Am Chem Soc 132:32-3
Naivar, Mark A; Wilder, Mark E; Habbersett, Robert C et al. (2009) Development of small and inexpensive digital data acquisition systems using a microcontroller-based approach. Cytometry A 75:979-89
Sebba, David S; Watson, Dakota A; Nolan, John P (2009) High throughput single nanoparticle spectroscopy. ACS Nano 3:1477-84
Perez-Pineiro, Rolando; Dai, Sheng; Alvarez-Puebla, Ramon et al. (2009) Synthesis of Sulfur-Containing Aryl and Heteroaryl Vinyls via Suzuki-Miyaura Cross-Coupling for the Preparation of SERS-Active Polymers. Tetrahedron Lett 50:5467-5469

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