Therapeutic proteins provide unique treatments for numerous human diseases and disorders including cancer, multiple sclerosis, hemophilia, rheumatoid arthritis, Crohn's disease and diabetes. Unfortunately, in significant fractions of patients receiving therapeutic protein products (up to 50% or higher), efficacy is lost due to immune response to the product. Immunogenicity may be stimulated by the presence of protein aggregates. In this project, we will use a hierarchical approach that uses advanced computer simulations combined with state-of-the art experiments to provide detailed, molecular-level information on the mechanisms by which protein aggregation occurs. Experiments will utilize synthetic proteins with designed chemical characteristics, model therapeutic proteins in murine models and commercial therapeutic proteins. Protein aggregates and pathways for their formation will be analyzed using a wide array of experimental techniques including single-molecule total internal reflectance fluorescence-Forster resonant energy transfer, small angle neutron scattering, small angle x-ray scattering micro-flow imaging, surface micro-rheology, chromatography, circular dichroism spectroscopy and infrared spectroscopy. A major focus of this renewal proposal will be the extension of our current work on protein aggregation in homogeneous solutions to protein aggregation that occurs due to interactions with interfaces such as those found in delivery devices (e.g., syringes, IV bags, IV lines, and delivery pumps).

Public Health Relevance

Aggregates formed within formulations of therapeutic proteins pose a risk for patient safety by increasing risk for adverse immune responses. By understanding the fundamental mechanisms that lead to therapeutic protein aggregation and associated immune responses, interdiction strategies can be developed that enhance the safety and efficacy of this valuable class of therapeutic molecules.

National Institute of Health (NIH)
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Research Project (R01)
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Macromolecular Structure and Function A Study Section (MSFA)
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Tucker, Jessica
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University of Colorado at Boulder
Engineering (All Types)
Schools of Engineering
United States
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Randolph, Theodore W; Schiltz, Elise; Sederstrom, Donn et al. (2015) Do not drop: mechanical shock in vials causes cavitation, protein aggregation, and particle formation. J Pharm Sci 104:602-11
Fradkin, Amber Haynes; Mozziconacci, Olivier; Schöneich, Christian et al. (2014) UV photodegradation of murine growth hormone: chemical analysis and immunogenicity consequences. Eur J Pharm Biopharm 87:395-402
Weiss 4th, William F; Zhang, Aming; Ivanova, Magdalena I et al. (2014) Reduction of the C191-C220 disulfide of ?-chymotrypsinogen A reduces nucleation barriers for aggregation. Biophys Chem 185:79-87
Xu, Yemin; Grobelny, Pawel; Von Allmen, Alexander et al. (2014) Protein quantity on the air-solid interface determines degradation rates of human growth hormone in lyophilized samples. J Pharm Sci 103:1356-66
Devineni, Dilip; Gonschorek, Christoph; Cicerone, Marcus T et al. (2014) Storage stability of keratinocyte growth factor-2 in lyophilized formulations: effects of formulation physical properties and protein fraction at the solid-air interface. Eur J Pharm Biopharm 88:332-41
Christie, Merry; Torres, Raul M; Kedl, Ross M et al. (2014) Recombinant murine growth hormone particles are more immunogenic with intravenous than subcutaneous administration. J Pharm Sci 103:128-39
Wagoner, Victoria A; Cheon, Mookyung; Chang, Iksoo et al. (2014) Impact of sequence on the molecular assembly of short amyloid peptides. Proteins 82:1469-83
Levenson, Eric A; Kiick, Kristi L (2014) DNA-polymer conjugates for immune stimulation through Toll-like receptor 9 mediated pathways. Acta Biomater 10:1134-45
Roberts, Christopher J (2014) Therapeutic protein aggregation: mechanisms, design, and control. Trends Biotechnol 32:372-80
Roberts, Christopher J (2014) Protein aggregation and its impact on product quality. Curr Opin Biotechnol 30:211-7

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