Cardiovascular disease affects 80 million people in the US and is the leading cause of death. Significant limitations of current treatments necessitate the development of novel strategies. Cardiovascular tissue engineering is an emerging field focused on the development of biological substitutes to restore, maintain, or improve tissue function. Bioengineered 3-dimensional (3D) cardiac patches can be utilized clinically at the site of a myocardial infarction, promoting increased left ventricular contractile function. This study is focused on the fabrication of 3D heart muscle, using controlled stretch to support tissue development and testing the ability of cardiac patches to augment the function of infarcted myocardium. The proposed study is divided into three components. The first part of this study is focused on defining cell culture conditions to maximize the functional performance of 3D cardiac patches. The second part is focused on developing protocols to deliver controlled stretch to the 3D cardiac patches, in order to further increase the functional performance of cardiac patches. In the third part of this project, cardiac patches fabricated using optimized cell culture conditions and conditioned using stretch, will be implanted on the surface of infarcted hearts. The ability of the cardiac patches to augment the functional performance of failing myocardium will be studied, as a function of post-injury time and patch implantation time. Successful completion of this study will result in the development of 3D cardiac patches which will be functional closer to normal mammalian heart muscle and will also provide insight into the potential applicability of the cardiac patches in supporting functional recovery of failing myocardium.
Cardiovascular disorders remain a major medical challenge with an urgent need to develop novel therapeutic options. In this study, cardiac patches will be fabricated and tested as a platform to support the recovery of infracted hearts. Successful completion of this study will result in the development of platform technology and provide a novel treatment modality for heart failure patients, benefiting millions of patients around the world.
|Tao, Ze-Wei; Mohamed, Mohamed; Jacot, Jeffrey G et al. (2018) Bioengineering Cardiac Tissue Constructs With Adult Rat Cardiomyocytes. ASAIO J 64:e105-e114|
|Salazar, Betsy H; Hoffman, Kristopher A; Reddy, Anilkumar K et al. (2018) 16-Channel Flexible System to Measure Electrophysiological Properties of Bioengineered Hearts. Cardiovasc Eng Technol 9:94-104|
|Tao, Ze-Wei; Mohamed, Mohamed; Hogan, Matthew et al. (2017) Optimizing a spontaneously contracting heart tissue patch with rat neonatal cardiac cells on fibrin gel. J Tissue Eng Regen Med 11:153-163|
|Mohamed, Mohamed A; Islas, Jose F; Schwartz, Robert J et al. (2017) Electrical Stimulation of Artificial Heart Muscle: A Look Into the Electrophysiologic and Genetic Implications. ASAIO J 63:333-341|
|Patel, Nikita M; Birla, Ravi K (2016) Pulsatile flow conditioning of three-dimensional bioengineered cardiac ventricle. Biofabrication 9:015003|
|Patel, Nikita M; Yazdi, Iman K; Tasciotti, Ennio et al. (2016) Optimizing cell seeding and retention in a three-dimensional bioengineered cardiac ventricle: The two-stage cellularization model. Biotechnol Bioeng 113:2275-85|
|Patel, Nikita M; Tao, Ze-Wei; Mohamed, Mohamed A et al. (2015) Engineering 3D bio-artificial heart muscle: the acellular ventricular extracellular matrix model. ASAIO J 61:61-70|
|Hogan, Matthew; Mohamed, Mohamed; Tao, Ze-Wei et al. (2015) Establishing the framework to support bioartificial heart fabrication using fibrin-based three-dimensional artificial heart muscle. Artif Organs 39:165-71|
|Mohamed, Mohamed A; Hogan, Matt K; Patel, Nikita M et al. (2015) Establishing the Framework for Tissue Engineered Heart Pumps. Cardiovasc Eng Technol 6:220-9|
|Salazar, Betsy H; Reddy, Anilkumar K; Zewei Tao et al. (2015) 32-Channel System to Measure the Electrophysiological Properties of Bioengineered Cardiac Muscle. IEEE Trans Biomed Eng 62:1614-22|
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