Cell-based therapeutics hold promise to revolutionize medicine, being applicable to numerous disease states. Due to the complex nature of cell-based medicine, its transition from proof-of-concept to clinical use has been slow. A major factor limiting the translation of cell-based therapeutics is the difficulty in tracking the fate of the cells in vivo. Positron emission tomography (PET) is an imaging technology that allows in vivo monitoring in real time and in a non-invasive manner. The signal measured in PET comes from radioactive isotopes, such a fluorine-18, that undergoes positron emission. Thus, a molecule containing such an isotope - called a PET probe - that specifically accumulates inside the therapeutic cells would reveal their location, number, half-life, etc. An additional factor limiting the advancement of therapeutic cells to the clinic has been the concern of unforeseen side effects from this novel approach. A safety mechanism that would allow for the elimination of the cells would greatly diminish this concern. The long-term goal of this proposal is to develop a dual-purpose system that would allow, one, for the real-time tracking of therapeutic cells using PET, and two, for the elimination of the cells if needed. Specific accumulation of the PET probe inside the therapeutic cells is achieved when the probe is phosphorylated in such cells. Phosphorylation traps the molecule within the cell due since charged molecules cannot traverse cell membranes. We propose to insert the gene of a human nucleoside kinase to the therapeutic cells, with the requirement that the kinase would impart unique activity to cells that express it. Such a unique enzymatic activity of a PET reporter enzyme would allow to preferentially phosphorylate novel PET probes in the therapeutic cells. The field currently relies on non-human enzymes, such as HSV1-TK, for that unique enzymatic activity. However, as a PET reporter enzyme HSV1-TK has several drawbacks, foremost being immunogenic. To circumvent this, we will employ a modified version of human thymidine kinase 2 (TK2) as the source for the unique enzymatic activity. To differentiate this enzyme from endogenous TK2, we will perform enzyme engineering to supply us with TK2 variants with an activity profile different to that of wild type TK2, but that still do not elicit an immune response. The immediate goals of this proposal are to study the determinants of substrate specificity of TK2 by solving crystal structures of the kinase in complex with substrates (Aim 1), and to exploit this understanding for the design of TK2 variants with unique activity towards novel PET probes (Aim 2). We will test the ability to track cells expressing our TK2 variants in mouse models, and compare the TK2-system to the standard in the field using HSV1-TK. The results of this research, a non-immunogenic PET reporter enzyme with optimized activity with PET probes, will allow the in vivo real time tracking of therapeutic cells. This would have a dramatic impact on the transition of therapeutic cell approaches to the clinic.
The use of cells in therapy is hampered by the difficulty in tracking the location and fate of the cells given to the patient. For the in vivo real time imaging of therapeutic cells using positron emission tomography (PET), we will engineer a unique version of a human enzyme that will have the ability to activate novel PET probes. The human source of the enzyme will circumvent an immune response against the therapeutic cells. Thus, the PET probes activated by the engineered enzyme will reveal the fate of therapeutic cells, with minimal background signal from the patient's cells that do not contain this ability. This development will facilitate the transition of therapeutic cells to the clinic.
|Schalk, Amanda M; Antansijevic, Aleksandar; Caffrey, Michael et al. (2016) Experimental Data in Support of a Direct Displacement Mechanism for Type I/II L-Asparaginases. J Biol Chem 291:5088-100|
|Nguyen, Hien Anh; Su, Ying; Lavie, Arnon (2016) Structural Insight into Substrate Selectivity of Erwinia chrysanthemi L-asparaginase. Biochemistry 55:1246-53|
|Nguyen, Hien Anh; Su, Ying; Lavie, Arnon (2016) Design and Characterization of Erwinia Chrysanthemi l-Asparaginase Variants with Diminished l-Glutaminase Activity. J Biol Chem 291:17664-76|
|Nathanson, David A; Armijo, Amanda L; Tom, Michelle et al. (2014) Co-targeting of convergent nucleotide biosynthetic pathways for leukemia eradication. J Exp Med 211:473-86|
|Nomme, Julian; Murphy, Jennifer M; Su, Ying et al. (2014) Structural characterization of new deoxycytidine kinase inhibitors rationalizes the affinity-determining moieties of the molecules. Acta Crystallogr D Biol Crystallogr 70:68-78|
|Murphy, Jennifer M; Armijo, Amanda L; Nomme, Julian et al. (2013) Development of new deoxycytidine kinase inhibitors and noninvasive in vivo evaluation using positron emission tomography. J Med Chem 56:6696-708|
|Campbell, Dean O; Yaghoubi, Shahriar S; Su, Ying et al. (2012) Structure-guided engineering of human thymidine kinase 2 as a positron emission tomography reporter gene for enhanced phosphorylation of non-natural thymidine analog reporter probe. J Biol Chem 287:446-54|