Long-term biopreservation of cells and tissues has a broad impact in multiple fields including tissue engineering, regenerative medicine, stem cells, blood banking, animal strain preservation (biodiversity protection), clinical sample storage, transplantation medicine and in vitro drug testing. Vitrification (ice/crystal-free cryopreservatio) has emerged as a novel approach over traditional slow freezing methods. Although vitrification minimizes mechanical damage due to ice crystal nucleation, it suffers from toxicity due to high concentrations of cryoprotectant agents (CPAs). The current vitrification methods require extremely high levels of CPAs of up to 8.2 M that are cytotoxic and cause osmotic shock. Also, the lengthy manual processing steps of current vitrification methods add to the technical complexity, require highly trained technicians, and result in variations between users. For instance, low CPA-level vitrification has immense potential for the stem cells compared to other methods in preserving their functionality. Recently, we demonstrated that we can achieve vitrification at ultra-rapid freezing and thawing rates with as low as 1.5M CPA concentration. We are adapting this new knowledge to the vital needs of cell cryopreservation at the clinic includingdiscarded anonymous human oocytes. This proposal investigates a new experimental strategy to minimize the CPA concentrations and improve clinical outcomes using novel technologies (i.e., nanoliter droplet vitrification). These steps are facilitated by theoretical understanding o the underlying mechanisms governing vitrification. The expected outcome of this study is a closed-system platform technology with broad applications to human cell (e.g., hepatocytes, oocytes, sperm, stem cells), tissues (e.g., blood), micro-tissues (e.g., embryoid bodies, islets) covering areas of reproductive medicine, tissue engineering and regenerative medicine as well as to wild life preservation. These studies can also significantly impact the care of infertile couples and facilitate fertility preservation.
Long-term biopreservation of cells and tissues has a broad impact in multiple fields including tissue engineering; regenerative medicine; blood banking; wild life preservation (biodiversity protection); clinical sample storage; transplantation medicine; reproductive medicine and in vitro drug testing. This proposal creates a novel technology platform and a new strategy to minimize the CPA concentrations in vitrification (ice/crystal-free cryopreservation) and to improve clinical outcomes using novel nanoliter droplet technologies; significantly impacting the care of infertile couples and facilitating fertility preservation. The expected outcome of this study is a closed-system platform cryopreservation technology with broad applications to human cells (including oocytes; sperm; and stem cells); tissues (including blood); micro-tissues (including embryoid bodies; islets).
|Chen, Pu; Luo, Zhengyuan; Güven, Sinan et al. (2014) Microscale assembly directed by liquid-based template. Adv Mater 26:5936-41|
|El Assal, Rami; Guven, Sinan; Gurkan, Umut Atakan et al. (2014) Bio-inspired cryo-ink preserves red blood cell phenotype and function during nanoliter vitrification. Adv Mater 26:5815-22|
|Tasoglu, S; Diller, E; Guven, S et al. (2014) Untethered micro-robotic coding of three-dimensional material composition. Nat Commun 5:3124|
|Asghar, Waseem; Velasco, Vanessa; Kingsley, James L et al. (2014) Selection of functional human sperm with higher DNA integrity and fewer reactive oxygen species. Adv Healthc Mater 3:1671-9|
|Tasoglu, S; Yu, C H; Gungordu, H I et al. (2014) Guided and magnetic self-assembly of tunable magnetoceptive gels. Nat Commun 5:4702|
|Asghar, Waseem; El Assal, Rami; Shafiee, Hadi et al. (2014) Preserving human cells for regenerative, reproductive, and transfusion medicine. Biotechnol J 9:895-903|
|Rizvi, Imran; Gurkan, Umut A; Tasoglu, Savas et al. (2013) Flow induces epithelial-mesenchymal transition, cellular heterogeneity and biomarker modulation in 3D ovarian cancer nodules. Proc Natl Acad Sci U S A 110:E1974-83|
|Tasoglu, Savas; Safaee, Hooman; Zhang, Xiaohui et al. (2013) Exhaustion of racing sperm in nature-mimicking microfluidic channels during sorting. Small 9:3374-84|
|Tasoglu, Savas; Demirci, Utkan (2013) Bioprinting for stem cell research. Trends Biotechnol 31:10-9|
|Tasoglu, Savas; Gurkan, Umut Atakan; Wang, Shuqi et al. (2013) Manipulating biological agents and cells in micro-scale volumes for applications in medicine. Chem Soc Rev 42:5788-808|