Exposure of the skin to ultraviolet radiation and to environmental toxic compounds that produce oxidative damage have numerous negative health consequences, among which the development of skin cancer is one of the most serious. The mechanisms by which these agents interact with the epidermis and by which the epidermal cells respond to damage are incompletely understood. Of crucial interest is the identity of cellular molecules that sense damage from environmental stressors, the intracellular signaling pathways by which the damage is conveyed, and the mechanisms that determine how cells choose between survival and death. During the previous grant cycle the applicant identified the 28S rRNA of ribosomes as a molecular sensor for stress induced by ultraviolet B radiation and other toxic molecules. Signals from ribosomes are quickly conveyed through pathways that activate the stress-activated protein kinases (SAPK) and appear to be involved in many of the crucial pathways that determine survival or apoptosis. Agents that produce oxidative damage do not mediate stress signals via 28S rRNA, and neither ultraviolet B or C radiation activates the SAPK (JNK and p38 MAPK) by inducing oxidative stress in cells. Recently the applicants have discovered that in primary normal keratinocytes the activation of the SAPK cascade by either arsenite, an oxidative stressor, or ultraviolet B radiation leads to an immediate and coordinate dephosphorylation and inactivation of ERK, a kinase family member that appears to be involved in regulation of cell proliferation and survival. The applicants' evidence indicates that the rapid dephosphorylation of ERK results from a stress-induced activation of a pre-existing phosphatase.
The aims of this renewal application are to identify in keratinocytes the phosphatase responsible for the dephosphorylation of ERK in response to ultraviolet B radiation and arsenite and to identify the mode of regulation of the stress-activated ERK phosphatase. The applicants will also determine whether members of the SAPK signaling cascade are responsible for the activation of the ERK phosphatase. Finally, they propose to elucidate the roles of JNK, p38 MAP, and ERK in apoptotic and prosurvival responses of human keratinocytes to ultraviolet B radiation and oxidative stress.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Research Project (R01)
Project #
5R01ES008456-06
Application #
6518118
Study Section
Chemical Pathology Study Section (CPA)
Program Officer
Mastin, Patrick
Project Start
1996-12-01
Project End
2006-02-28
Budget Start
2002-03-25
Budget End
2003-02-28
Support Year
6
Fiscal Year
2002
Total Cost
$377,500
Indirect Cost
Name
Oregon Health and Science University
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
009584210
City
Portland
State
OR
Country
United States
Zip Code
97239
Iordanov, M S; Kirsch, J D; Ryabinina, O P et al. (2005) Recruitment of TRADD, FADD, and caspase 8 to double-stranded RNA-triggered death inducing signaling complexes (dsRNA-DISCs). Apoptosis 10:167-76
Iordanov, M S; Ryabinina, O P; Schneider, P et al. (2005) Two mechanisms of caspase 9 processing in double-stranded RNA- and virus-triggered apoptosis. Apoptosis 10:153-66
Iordanov, Mihail S; Sundholm, Aaron J; Simpson, Eric L et al. (2005) Cell death-induced activation of epidermal growth factor receptor in keratinocytes: implications for restricting epidermal damage in dermatitis. J Invest Dermatol 125:134-42
Iordanov, Mihail S; Choi, Remy J; Ryabinina, Olga P et al. (2002) The UV (Ribotoxic) stress response of human keratinocytes involves the unexpected uncoupling of the Ras-extracellular signal-regulated kinase signaling cascade from the activated epidermal growth factor receptor. Mol Cell Biol 22:5380-94
Newton, D L; Hansen, H J; Liu, H et al. (2001) Specifically targeting the CD22 receptor of human B-cell lymphomas with RNA damaging agents. Crit Rev Oncol Hematol 39:79-86
Iordanov, M S; Wong, J; Bell, J C et al. (2001) Activation of NF-kappaB by double-stranded RNA (dsRNA) in the absence of protein kinase R and RNase L demonstrates the existence of two separate dsRNA-triggered antiviral programs. Mol Cell Biol 21:61-72
Iordanov, M S; Paranjape, J M; Zhou, A et al. (2000) Activation of p38 mitogen-activated protein kinase and c-Jun NH(2)-terminal kinase by double-stranded RNA and encephalomyocarditis virus: involvement of RNase L, protein kinase R, and alternative pathways. Mol Cell Biol 20:617-27
Iordanov, M S; Ryabinina, O P; Wong, J et al. (2000) Molecular determinants of apoptosis induced by the cytotoxic ribonuclease onconase: evidence for cytotoxic mechanisms different from inhibition of protein synthesis. Cancer Res 60:1983-94
Iordanov, M S; Wong, J; Newton, D L et al. (2000) Differential requirement for the stress-activated protein kinase/c-Jun NH(2)-terminal kinase in RNAdamage-induced apoptosis in primary and in immortalized fibroblasts. Mol Cell Biol Res Commun 4:122-8
Keller, D; Zeng, X; Li, X et al. (1999) The p38MAPK inhibitor SB203580 alleviates ultraviolet-induced phosphorylation at serine 389 but not serine 15 and activation of p53. Biochem Biophys Res Commun 261:464-71

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