Epidemiological data support a causal link between exposure to elevated levels of particulate matter (PM) and increased lower respiratory tract infections (LRTIs) in children. During the H1N1 influenza (Flu) pandemic, exposure to PM was a potential contributing factor to the disparity in the increased levels of H1N1-induced morbidity and mortality observed in Mexico and the United States. Interestingly, the risk of LRTIs due to PM exposure is highest in infants. Despite strong evidence associating PM exposure and LRTI susceptibility, morbidity, and mortality in infants;there is very little research on this subject nd the mechanisms underlying this phenomenon are unknown. We have developed a novel neonatal (<7 d of age) rodent model for studying PM exposures, which we apply here to understand the effects of PM on enhanced susceptibility to LRTI and LRTI-mediated disease severity. Our data show that age of exposure to PM is important in predicting LRTI disease sequela and that infant exposure to PM initiated several events that may explain the epidemiological data. First, exposure of neonatal mice to PM results in epithelial disruption, which allows for enhanced access of respiratory viruses to the lower airways. Second, adaptive immune responses following PM exposure in neonates are suppressive in nature (i.e. increased IL10 and Treg cells and decreased Th1, Tc1, and Th17 cell numbers) and not protective. The end result is enhanced severity of Flu-mediated disease as evidenced by increased pulmonary viral loads and mortality in neonatal mice infected following exposure to PM. Our data further suggest that epithelial signals either cell associated or secreted (i.e. epimmunome) are used to direct this aberrant immune response to Flu. Thus, we hypothesize that exposure to PM during infancy increases the risk for and severity of infectious respiratory disease through a process involving epithelial disruption and alteration of the epimmunome.
Our specific aims will address the following important questions. 1) How does neonatal exposure to PM increase susceptibility to Flu infection and enhanced disease? 2) Is epithelial disruption following neonatal exposure to PM responsible for enhanced Flu susceptibility? 3) Does neonatal exposure to PM enhance Flu susceptibility by suppressing the pulmonary immune response? Completion of these studies will provide us with an understanding of the molecular signaling events between injured epithelial cells and DCs crucial to understand Flu pathogenesis in PM exposed infants and to identify pharmacologic targets for the treatment of environmentally-induced asthma exacerbations due to LRTI.

Public Health Relevance

Elevated levels of PM increase risk of infant mortality from lower respiratory tract infections such as influenza;and yet, few studies have tried to understand the mechanisms responsible for increased risk for LRTIs in this population following exposure to PM. Despite these facts, there is an urgent need for research in this area to understand the public health risks and develop therapeutic interventions. The concepts established here will have important implications for understanding mechanisms of PM-mediated airway disease and for understanding mechanisms of the epimmunome relevant to reducing morbidity and mortality associated with PM-exacerbated LRTI.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Research Project (R01)
Project #
7R01ES015050-08
Application #
8662987
Study Section
Lung Injury, Repair, and Remodeling Study Section (LIRR)
Program Officer
Nadadur, Srikanth
Project Start
2006-09-01
Project End
2017-10-31
Budget Start
2013-05-16
Budget End
2013-10-31
Support Year
8
Fiscal Year
2013
Total Cost
$299,950
Indirect Cost
$99,950
Name
University of Tennessee Health Science Center
Department
Pediatrics
Type
Schools of Medicine
DUNS #
941884009
City
Memphis
State
TN
Country
United States
Zip Code
38163
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