Due to the their widespread use and stability, sediments contaminated with persistent organic pollutants (POPs) such as polychlorinated biphenyls, chlorobenzenes and dioxins have been an environmental concern for several decades. In addition to perturbing the benthic community, these compounds biomagnify in the food chain through accumulation in the fatty tissue of animals, such as fish and marine mammals, which can eventually affect humans if consumed. Exposure to halogenated POPs by humans can lead to dermal toxicity, teratotoxicity, endocrine effects, hepatotoxicity, immunotoxicity, and carcinogenesis. Although chemically stable in the environment these highly chlorinated compounds are susceptible to degradation once most of their chlorines are removed. The initial dechlorination is catalyzed in the anaerobic environment by microbial reductive respiration;the products of this initial microbial process can then be degraded and detoxified by oxygen respiring microorganisms. The limitations of these processes are: 1) anaerobic dechlorination is often slow and incomplete;2) aerobic degradation is inhibited by limited availability of oxygen in the anaerobic sediment regions where these compounds persist. The Principal Investigators propose to complete the anaerobic process to unflanked di- through tetrachlorinated by in situ bioaugmention with dechlorinating microorganisms followed by application of low current hydrolysis to provide a constant level of oxygen for the complete degradation of the chlorinated compounds. The innovative aspects of this approach include the use of dechlorinating species with specific activities to direct the anaerobic dechorination pathways, a unique process for scaling up dehalogenating inoculum without co-release of toxic chlorinated compounds, electrolysis of water for maintaining constant oxygen levels during aerobic degradation and high throughput molecular assays for monitoring microbial communities. This integrated approach will optimize both anaerobic and aerobic processes to achieve complete in situ detoxification of chlorinated compounds through mineralization to small molecular weight metabolites and carbon dioxide. Implementation of a tractable in situ detoxification process to organohalide impacted sites will mitigate exposure to the food chain and subsequent exposure risks to the general public.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Research Project (R01)
Project #
5R01ES016197-03
Application #
7761262
Study Section
Special Emphasis Panel (ZES1-SET-D (R1))
Program Officer
Henry, Heather F
Project Start
2008-02-15
Project End
2012-01-31
Budget Start
2010-02-01
Budget End
2012-01-31
Support Year
3
Fiscal Year
2010
Total Cost
$295,993
Indirect Cost
Name
Medical University of South Carolina
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
183710748
City
Charleston
State
SC
Country
United States
Zip Code
29425
Lombard, Nathalie J; Ghosh, Upal; Kjellerup, Birthe V et al. (2014) Kinetics and threshold level of 2,3,4,5-tetrachlorobiphenyl dechlorination by an organohalide respiring bacterium. Environ Sci Technol 48:4353-60
Sowers, Kevin R; May, Harold D (2013) In situ treatment of PCBs by anaerobic microbial dechlorination in aquatic sediment: are we there yet? Curr Opin Biotechnol 24:482-8
Chun, Chan Lan; Payne, Rayford B; Sowers, Kevin R et al. (2013) Electrical stimulation of microbial PCB degradation in sediment. Water Res 47:141-52
Payne, Rayford B; Fagervold, Sonja K; May, Harold D et al. (2013) Remediation of polychlorinated biphenyl impacted sediment by concurrent bioaugmentation with anaerobic halorespiring and aerobic degrading bacteria. Environ Sci Technol 47:3807-15
Lombard, Nathalie; Prestat, Emmanuel; van Elsas, Jan Dirk et al. (2011) Soil-specific limitations for access and analysis of soil microbial communities by metagenomics. FEMS Microbiol Ecol 78:31-49
Payne, Rayford B; May, Harold D; Sowers, Kevin R (2011) Enhanced reductive dechlorination of polychlorinated biphenyl impacted sediment by bioaugmentation with a dehalorespiring bacterium. Environ Sci Technol 45:8772-9