Capillaries are of central importance in the three most common retinal vascular diseases in man: diabetic retinopathy (DR), hypertensive retinopathy (HR) and vein occlusion (VO). The cells forming the capillary wall, endothelial cells and pericytes, contain a structural apparatus which involves actin and fibronectin (FN) and suggests capillary contractile function. During DR, HR and VO the capillary contractile apparatus is subject to stress and may structurally change. Thus, our AIM I is to evaluate: 1. The content and distribution of actin in capillary pericytes and endothelial cells using the myosin subfragment-1 (S-1) technique for actin staining and quantitative morphometry. 2. The coexistence and coextensions of actin and FN (=fibronexus, FNX) in capillary pericytes localized and endothelial cells using mainly EM immunocytochemistry (immunoferritin). I,1. and I,2. are to be examined in 4 model situations: DR in rats and human, HR in rats, VO in monkeys. Preretinal membranes (PRM's) are part of proliferative vitreoretinopathy (PVR) an important cause of human blindness. PRM's are intraocular scar tissue that shrinks causing retinal detachment and atrophy. The scar tissue develops by proliferation and migration of a variety of cells whose identity is not fully known. Contraction of PRM's seems to be a cell-mediated event and may involve actin and FNX relationships. Thus, our AIM II is to evaluate: 1. The identity of proliferating and migrating cells during the development of PRM's using LM immunohistochemistry (immunoperoxidase) with markers for glial cells (GFAP), epithelial cells (prekeratin and keratin) and endothelial cells (Factor VIII-related antigen). 2. The presence of FNX relationships by both LM and EM immunocytochemistry. II,1. and II,2. are to be examined in 3 model situations: PRM's produced by injection of colloidal carbon into the vitreous of rabbits and monkeys, and spontaneous PRM's developing during human PVR.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY001634-13
Application #
3256066
Study Section
Visual Sciences B Study Section (VISB)
Project Start
1979-05-01
Project End
1990-04-30
Budget Start
1988-05-01
Budget End
1989-04-30
Support Year
13
Fiscal Year
1988
Total Cost
Indirect Cost
Name
University of Wisconsin Madison
Department
Type
Schools of Medicine
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
Tamm, E R; Rohen, J W; Schmidt, K et al. (1997) Superior cervical ganglionectomy in monkeys: light and electron microscopy of the anterior eye segment. Exp Eye Res 65:31-43
Brandt, C R; Pumfery, A M; Micales, B et al. (1994) Renin mRNA is synthesized locally in rat ocular tissues. Curr Eye Res 13:755-63
Sherman, D D; Gonnering, R S; Wallow, I H et al. (1993) Identification of orbital lymphatics: enzyme histochemical light microscopic and electron microscopic studies. Ophthal Plast Reconstr Surg 9:153-69
Stasior, G O; Lemke, B N; Wallow, I H et al. (1993) Levator aponeurosis elastic fiber network. Ophthal Plast Reconstr Surg 9:1-10
Wallow, I H; Bindley, C D; Reboussin, D M et al. (1993) Systemic hypertension produces pericyte changes in retinal capillaries. Invest Ophthalmol Vis Sci 34:420-30
Rutledge, B K; Wallow, I H; Poulsen, G L (1993) Sub-pigment epithelial membranes after photocoagulation for diabetic macular edema. Arch Ophthalmol 111:608-13
Wallow, I H; Sramek, S J; Bindley, C D et al. (1993) Ocular renin angiotensin: EM immunocytochemical localization of prorenin. Curr Eye Res 12:945-50
Sramek, S J; Wallow, I H; Tewksbury, D A et al. (1992) An ocular renin-angiotensin system. Immunohistochemistry of angiotensinogen. Invest Ophthalmol Vis Sci 33:1627-32
Lyon, D B; Lemke, B N; Wallow, I H et al. (1992) Sympathetic nerve anatomy in the cavernous sinus and retrobulbar orbit of the cynomolgus monkey. Ophthal Plast Reconstr Surg 8:1-12
Wallow, I H; Bindley, C D; Linton, K L et al. (1991) Pericyte changes in branch retinal vein occlusion. Invest Ophthalmol Vis Sci 32:1455-63

Showing the most recent 10 out of 29 publications