Abstract

This research is an investigation of synaptic interactions in the retina of the eye. More specifically, this research will concentrate on synaptic processing in the outer plexiform layer with an emphasis on identifying the synaptic transmitter substance released by photoreceptors and determining the actions of this transmitter. This study will use a newly developed technique called noise analysis. This analysis will allow characterization of how the postsynaptic channels in bipolar cells are affected by the photoreceptor transmitter. Putative transmitters will be superfused onto the retina and the noise analysis technique will again be used to characterize properties in bipolar cells. Comparing the properties of the channels under the normal and perfused states will allow us to identify the natural transmitter. A second part of this study will involve the fabrication of substrate specific electrodes which will be employed to measure directly the release of neurotransmitter from retinal neurons. This technique involves the use of pH or NH+ detectors coated with a gelatin which is embedded with an enzyme that degrades or hydrolyzes the neurotransmitter producing NH+ or H+ ions.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY001869-09
Application #
3256266
Study Section
(SSS)
Project Start
1977-02-01
Project End
1988-01-31
Budget Start
1985-02-01
Budget End
1986-01-31
Support Year
9
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of California San Francisco
Department
Type
Schools of Medicine
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143

  Publications

Delwig, Anton; Chaney, Shawnta Y; Bertke, Andrea S et al. (2018) Melanopsin expression in the cornea. Vis Neurosci 35:E004
Delwig, Anton; Larsen, DeLaine D; Yasumura, Douglas et al. (2016) Retinofugal Projections from Melanopsin-Expressing Retinal Ganglion Cells Revealed by Intraocular Injections of Cre-Dependent Virus. PLoS One 11:e0149501
Rao, Sujata; Chun, Christina; Fan, Jieqing et al. (2013) A direct and melanopsin-dependent fetal light response regulates mouse eye development. Nature 494:243-6
Delwig, Anton; Majumdar, Sriparna; Ahern, Kelly et al. (2013) Glutamatergic neurotransmission from melanopsin retinal ganglion cells is required for neonatal photoaversion but not adult pupillary light reflex. PLoS One 8:e83974
Delwig, Anton; Logan, Anne M; Copenhagen, David R et al. (2012) Light evokes melanopsin-dependent vocalization and neural activation associated with aversive experience in neonatal mice. PLoS One 7:e43787
Chen, Albert I; Nguyen, Cindy N; Copenhagen, David R et al. (2011) TrkB (tropomyosin-related kinase B) controls the assembly and maintenance of GABAergic synapses in the cerebellar cortex. J Neurosci 31:2769-80
Yoshida, Miho; Feng, Liang; Grimbert, Francois et al. (2011) Overexpression of neurotrophin-3 stimulates a second wave of dopaminergic amacrine cell genesis after birth in the mouse retina. J Neurosci 31:12663-73
Johnson, Juliette; Wu, Vincent; Donovan, Michael et al. (2010) Melanopsin-dependent light avoidance in neonatal mice. Proc Natl Acad Sci U S A 107:17374-8
Liu, Xiaorong; Robinson, Michael L; Schreiber, Ann Marie et al. (2009) Regulation of neonatal development of retinal ganglion cell dendrites by neurotrophin-3 overexpression. J Comp Neurol 514:449-58
Blankenship, Aaron G; Ford, Kevin J; Johnson, Juliette et al. (2009) Synaptic and extrasynaptic factors governing glutamatergic retinal waves. Neuron 62:230-41

Showing the most recent 10 out of 46 publications