Abstract

The proposed work will use a combination of immunocytochemical techniques to characterize the sites of action and relationships of receptors responsive to glutamate, the major excitatory neurotransmitter within the retina.
The specific aims are: (1) the localization of GluR subunits to cellular and subcellular sites, using light and electron microscopic immunocytochemistry to identify the cells in the cat retina that express subunits of the AMPA, kainate, NMDA and metabotropic types of GluR and to determine the relationship of these receptors to synaptic specializations; (2) comparison of the receptor distribution with the synaptic relationships of defined subpopulations of retinal neurons, using double-label techniques to compare the distribution of GluRs with a variety of neurochemical markers including neurotransmitters, synthetic enzymes, Ca2+-binding proteins, and second messengers, with specific attention being given to how these relationships correlate with the physiological properties of specific cells; and (3) comparison of GluR distribution with Ca2+-binding proteins and Ca2+-dependent second messengers, using double-labeled tissues to determine whether the retinal localizations are consistent with a role for Ca2+-binding proteins and second messengers in regulation or implementation of the functions of GluR subunits.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY002267-20
Application #
2838249
Study Section
Visual Sciences C Study Section (VISC)
Project Start
1978-12-01
Project End
2000-11-30
Budget Start
1998-12-01
Budget End
1999-11-30
Support Year
20
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
Wayne State University
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
City
Detroit
State
MI
Country
United States
Zip Code
48202

  Publications

Fyk-Kolodziej, Bozena; Pourcho, Roberta G (2007) Differential distribution of hyperpolarization-activated and cyclic nucleotide-gated channels in cone bipolar cells of the rat retina. J Comp Neurol 501:891-903
Fyk-Kolodziej, Bozena; Dzhagaryan, Arturik; Qin, Pu et al. (2004) Immunocytochemical localization of three vesicular glutamate transporters in the cat retina. J Comp Neurol 475:518-30
Fyk-Kolodziej, Bozena; Qin, Pu; Dzhagaryan, Arturik et al. (2004) Differential cellular and subcellular distribution of glutamate transporters in the cat retina. Vis Neurosci 21:551-65
Fyk-Kolodziej, Bozena; Qin, Pu; Pourcho, Roberta G (2003) Identification of a cone bipolar cell in cat retina which has input from both rod and cone photoreceptors. J Comp Neurol 464:104-13
Winkler, Barry S; Pourcho, Roberta G; Starnes, Catherine et al. (2003) Metabolic mapping in mammalian retina: a biochemical and 3H-2-deoxyglucose autoradiographic study. Exp Eye Res 77:327-37
Pourcho, Roberta G; Qin, Pu; Goebel, Dennis J et al. (2002) Agonist-stimulated cobalt uptake provides selective visualization of neurons expressing AMPA- or kainate-type glutamate receptors in the retina. J Comp Neurol 454:341-9
Fyk-Kolodziej, Bozena; Cai, Wenhui; Pourcho, Roberta G (2002) Distribution of protein kinase C isoforms in the cat retina. Vis Neurosci 19:549-62
Qin, P; Pourcho, R G (2001) Immunocytochemical localization of kainate-selective glutamate receptor subunits GluR5, GluR6, and GluR7 in the cat retina. Brain Res 890:211-21
Pourcho, R G; Qin, P; Goebel, D J (2001) Cellular and subcellular distribution of NMDA receptor subunit NR2B in the retina. J Comp Neurol 433:75-85
Cai, W; Pourcho, R G (1999) Localization of metabotropic glutamate receptors mGluR1alpha and mGluR2/3 in the cat retina. J Comp Neurol 407:427-37

Showing the most recent 10 out of 36 publications