The long-term scientific goal of the research project is to understand the molecular mechanism underlying Drosophila phototransduction. Drosophila photoreceptor cells are photosensitive neurons with a phototransduction cascade similar to that of the Retinal Ganglion Cells (RGC), which contain the photopigment melanopsin, thus being a """"""""Rosetta Stone"""""""" for RGC and other photosensitive neurons. Both Drosophila and presumably the light sensitive RGC are characterized by bi-stable rhodopsin that initiates phototranduction with Transient Receptor Potential (TRP) channels as the final target.
The specific aims are: i) to elucidate the mechanisms that make the photoreceptor rhabdomere a signaling compartment as a strategy to optimize function. The unknown functional properties of the Ca2+ binding protein, calphotin, will be studied. To this end calphotin levels will be modulated in vivo by overexpression or by RNAi and the physiological consequences will be studied. Moreover, we will examine the putative role of calphotin as a dynamic barrier controlling the free diffusion of Ca2+ between the rhabdomere and the cell body. ii) To study the unknown mechanism underlying light dependent translocation of the TRP-like (TRPL) channel. We will use TRP-TRPL chimeras expressed in the living fly eye to study the effects of various domains of the immobile TRP on translocation of TRPL. Since TRP but not TRPL binds to the INAD scaffold protein, the chimeras will allow elucidating new roles for INAD in phototransduction and whether or not TRP and TRPL form heteromultimeres. iii) To elucidate the unknown mechanism of TRP and TRPL activation by light. Potentially, a phospholipase C (PLC) - mediated phototransduction can activate the channels by at least three putative mechanisms: by second messengers, by removal of inhibition and by change in membrane curvature. More specifically: (a) Activation by the second messengers Inositol 1,4,5 trisphosphate (InsP3), Diacylglycerol (DAG) and subsequently Polyunsaturated Fatty Acids (PUFA) due to hydrolysis of DAG. (b) Activation by a decrease in the concentration of PIP2, thereby removing its inhibitory effect on the channels. (c) Activation by conversion of PIP2 to DAG or PUFA causing membrane lipid packing modifications that changes the curvature of the plasma membrane and thereby activate the channels in a manner similar to activation of mechanosensitive channels. We will examine these possibilities in expression system of Drosophila cell-line which express TRPL and in the native photoreceptor cells by inducing PIP2 hydrolysis without activation of PLC under conditions that change, or do not change membrane curvature. The activation mechanism of the putative mechanosensitive and Ca2+ permeable TRP channel will be used to acquire insights on the involvement of pathological activation of mammalian TRP channels in retinal degeneration and glaucoma.

Public Health Relevance

A powerful approach to identify potentially important proteins for mammalian retinal cells is to look for homologue proteins that have been identified in Drosophila by genetic screens, leading to isolation of mutants defective in retinal processes. Retinal degeneration or glaucoma, which leads by unknown ways to photoreceptor degeneration and apoptosis of retinal ganglion cells (RGC) respectively, may Involve Ca2+ permeable TRP channels activated by mechanical stretch and hypoxia like the Drosophila channels. Therefore, studies on Drosophila TRP and TRPL channels are likely to be relevant for understanding retinal degeneration and mechanisms underlying the damage to RGC by glaucoma.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY003529-31
Application #
8128475
Study Section
Biology and Diseases of the Posterior Eye Study Section (BDPE)
Program Officer
Neuhold, Lisa
Project Start
1981-09-01
Project End
2013-08-31
Budget Start
2011-09-01
Budget End
2013-08-31
Support Year
31
Fiscal Year
2011
Total Cost
$160,722
Indirect Cost
Name
Hebrew University of Jerusalem
Department
Type
DUNS #
600044978
City
Jerusalem
State
Country
Israel
Zip Code
91904
Voolstra, Olaf; Rhodes-Mordov, Elisheva; Katz, Ben et al. (2017) The Phosphorylation State of the Drosophila TRP Channel Modulates the Frequency Response to Oscillating Light In Vivo. J Neurosci 37:4213-4224
Weiss, Shirley; Minke, Baruch (2015) A new genetic model for calcium induced autophagy and ER-stress in Drosophila photoreceptor cells. Channels (Austin) 9:14-20
Kohn, Elkana; Katz, Ben; Yasin, Bushra et al. (2015) Functional cooperation between the IP3 receptor and phospholipase C secures the high sensitivity to light of Drosophila photoreceptors in vivo. J Neurosci 35:2530-46
Katz, Ben; Oberacker, Tina; Richter, David et al. (2013) Drosophila TRP and TRPL are assembled as homomultimeric channels in vivo. J Cell Sci 126:3121-33
Lev, Shaya; Katz, Ben; Minke, Baruch (2012) The activity of the TRP-like channel depends on its expression system. Channels (Austin) 6:86-93
Lev, Shaya; Katz, Ben; Tzarfaty, Vered et al. (2012) Signal-dependent hydrolysis of phosphatidylinositol 4,5-bisphosphate without activation of phospholipase C: implications on gating of Drosophila TRPL (transient receptor potential-like) channel. J Biol Chem 287:1436-47
Katz, Ben; Minke, Baruch (2012) Phospholipase C-mediated suppression of dark noise enables single-photon detection in Drosophila photoreceptors. J Neurosci 32:2722-33
Weiss, Shirley; Kohn, Elkana; Dadon, Daniela et al. (2012) Compartmentalization and Ca2+ buffering are essential for prevention of light-induced retinal degeneration. J Neurosci 32:14696-708
Richter, David; Katz, Ben; Oberacker, Tina et al. (2011) Translocation of the Drosophila transient receptor potential-like (TRPL) channel requires both the N- and C-terminal regions together with sustained Ca2+ entry. J Biol Chem 286:34234-43
Zeevi, David A; Lev, Shaya; Frumkin, Ayala et al. (2010) Heteromultimeric TRPML channel assemblies play a crucial role in the regulation of cell viability models and starvation-induced autophagy. J Cell Sci 123:3112-24

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