The objectives of the proposed research are to establish animal models of different types of orbital lymphoproliferative and granulomatous inflamation, in order to elucidate the basic immunologic mechanisms in the induction of these ocular diseases, and to develop methods to prevent or suppress them by manipulating the immune system. An experimental model of immunogenic pseudotumor of the orbit in inbred rats has recently been developed. This model involves the administration of insoluble beads coated with antigen in the orbital tissue of previously immunized rats. The resulting chronic granuloma was shown to be a T cell-mediated response.
The specific aim of the proposed study is to explore methods to prevent or suppress such harmful inflammation, using syngeneic cell-bound antigens to induce suppressor cell activity, and T lymphoblasts to confer protective anti-idiotypic immunity. An experimental model of pseudotumorous dacryoadenitis has also been developed which involves the induction of a spontaneous lymphoproliferative disease by immunizing rats to an organ-specific antigen of the lacrimal gland. The resulting lesions have many features in common with those present in human disease. Efforts will be made to isolate, purify, chemically characterize, and topographically localize the autoantigen responsible, to aid in better understanding the basic pathogenetic mechanisms of this autoimmune disease. Using in vitro cytotoxicity assays and passive transfer experiments, attempts will be made to define the immunological mechanisms that cause this lymphoproliferative disease. Specifically, these studies will define more precisely the nature of the damaging autoimmune reactions participating in this ocular disease, to characterize the types of cells that trigger and mediate the inflammatory response, and to define the role of T cells or B cells in this autoallergic reaction.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY004444-06
Application #
3258865
Study Section
Visual Sciences A Study Section (VISA)
Project Start
1982-08-01
Project End
1988-07-31
Budget Start
1987-08-01
Budget End
1988-07-31
Support Year
6
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Johns Hopkins University
Department
Type
Schools of Medicine
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218
Liu, S H; Zhou, D H; Gottsch, J D et al. (1993) Treatment of experimental autoimmune dacryoadenitis with cyclosporin A. Clin Immunol Immunopathol 67:78-83
Liu, S H; Zhou, D H; Franklin, R M (1993) Lacrimal gland-derived lymphocyte proliferation potentiating factor. Invest Ophthalmol Vis Sci 34:650-7
Liu, S H; Zhou, D H; Hess, A D (1993) Adoptive transfer of experimental autoimmune dacryoadenitis in susceptible and resistant mice. Cell Immunol 150:311-20
Liu, S H; Zhou, D H (1992) Experimental autoimmune dacryoadenitis: purification and characterization of a lacrimal gland antigen. Invest Ophthalmol Vis Sci 33:2029-36
Gottsch, J D; Liu, S H; Stark, W J (1992) Mooren's ulcer and evidence of stromal graft rejection after penetrating keratoplasty. Am J Ophthalmol 113:412-7
Liu, S H; Prendergast, R A; Silverstein, A M (1987) Experimental autoimmune dacryoadenitis. I. Lacrimal gland disease in the rat. Invest Ophthalmol Vis Sci 28:270-5
Liu, S H; Sakai, F; Prendergast, R A et al. (1987) Experimental autoimmune dacryoadenitis. II. Harderian gland disease in the rat. Invest Ophthalmol Vis Sci 28:276-80
Liu, S H; Prendergast, R A; Silverstein, A M (1986) Experimental immunogenic granuloma of the orbit: transfer of granulomatous hypersensitivity with a subset of T lymphocytes. Invest Ophthalmol Vis Sci 27:70-6