Abstract

This proposal is a multidisciplinary approach to examine the hypothesis that the initial AU alters the BAB permeability, setting up stages for recurrence. The basis of this application will be to evaluate the tight junctions of the BAB following an intravitreal injection of endotoxin. The applicant will then look at the BAB repair in both the ciliary epithelium and iris vascular endothelium at several levels. The applicant has past experience with these techniques, including in vivo aqueous fluorophotometry and tracer localization with EM, computational modeling of the protein kinetics and confocal and electron microscopy localization of molecular probes relevant to the junctional reassembly. Freeze fracture will also be conducted. The last specific aim will be to use high resolution contract MRI to determine if anterior diffusion pathways exists in normal humans.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY004567-16
Application #
2710852
Study Section
Special Emphasis Panel (ZRG1-VISC (01))
Project Start
1983-01-01
Project End
2000-06-30
Budget Start
1998-07-01
Budget End
1999-06-30
Support Year
16
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
Boston University
Department
Ophthalmology
Type
Schools of Medicine
DUNS #
604483045
City
Boston
State
MA
Country
United States
Zip Code
02118

  Publications

Freddo, Thomas F (2013) A contemporary concept of the blood-aqueous barrier. Prog Retin Eye Res 32:181-95
Kolodny, N H; Goode, S T; Ryan, W et al. (2002) Evaluation of therapeutic effectiveness using MR imaging in a rabbit model of anterior uveitis. Exp Eye Res 74:483-91
Sonsino, Jeffrey; Gong, Haiyan; Wu, Ping et al. (2002) Co-localization of junction-associated proteins of the human blood--aqueous barrier: occludin, ZO-1 and F-actin. Exp Eye Res 74:123-9
Freddo, T F (2001) Shifting the paradigm of the blood-aqueous barrier. Exp Eye Res 73:581-92
Wu, P; Gong, H; Richman, R et al. (2000) Localization of occludin, ZO-1, and pan-cadherin in rabbit ciliary epithelium and iris vascular endothelium. Histochem Cell Biol 114:303-10
Krenzer, K L; Freddo, T F (1997) Cytokeratin expression in normal human bulbar conjunctiva obtained by impression cytology. Invest Ophthalmol Vis Sci 38:142-52
Gong, H; Johnson, M; Ye, W et al. (1997) The non-uniform distribution of albumin in human and bovine cornea. Exp Eye Res 65:747-56
Freddo, T F (1996) Ultrastructure of the iris. Microsc Res Tech 33:369-89
Kolodny, N H; Freddo, T F; Lawrence, B A et al. (1996) Contrast-enhanced magnetic resonance imaging confirmation of an anterior protein pathway in normal rabbit eyes. Invest Ophthalmol Vis Sci 37:1602-7
Johnson, M; Gong, H; Freddo, T F et al. (1993) Serum proteins and aqueous outflow resistance in bovine eyes. Invest Ophthalmol Vis Sci 34:3549-57

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