Abstract

The principal objectives of this proposal are to measure the serologic prevalence of toxocara canis infection in persons in the United States, to determine the epidemiologic pattern of infection with respect to demographic and geographic factors, and to assess the possible association of infection with visual deficits and ocular disease. For this purpose, we will perform the enzyme-linked immunosorbent assay (ELISA) on 8594 sera which were collected during the National Health and Nutrition Examination Survey of 1971-1973. Detailed demographic, medical, and ophthalmologic information is available for each of the participants. Another objective is to define the clinical, pathological, and immunological response of nonhuman primates with experimental ocular toxocariasis. Cynomolgus monkeys will be infected by either the gastrointestinal or intravitreal route to study the pathogenesis and pattern of larva-specific antibody production in the serum, aqueous and vitreous humor. This information will aid in the interpretation of serologic test results from children with suspected toxocaral visceral larva migrans or ocular toxocariasis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY005677-02
Application #
3261027
Study Section
Tropical Medicine and Parasitology Study Section (TMP)
Project Start
1984-05-01
Project End
1986-06-30
Budget Start
1985-03-01
Budget End
1986-06-30
Support Year
2
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Wisconsin Madison
Department
Type
Schools of Veterinary Medicine
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715

  Publications

Parsons, J C; Coffman, R L; Grieve, R B (1993) Antibody to interleukin 5 prevents blood and tissue eosinophilia but not liver trapping in murine larval toxocariasis. Parasite Immunol 15:501-8
Parsons, J C; Grieve, R B (1990) Kinetics of liver trapping of infective larvae in murine toxocariasis. J Parasitol 76:529-36
Grieve, R B (1990) Immunologic relevance of the cuticle and epicuticle of larval Dirofilaria immitis and Toxocara canis. Acta Trop 47:399-402
Parsons, J C; Grieve, R B (1990) Effect of egg dosage and host genotype on liver trapping in murine larval toxocariasis. J Parasitol 76:53-8
Parsons, J C; Bowman, D D; Grieve, R B (1989) Pathological and haematological responses of cats experimentally infected with Toxocara canis larvae. Int J Parasitol 19:479-88
Parsons, J C; Bowman, D D; Gillette, D M et al. (1988) Disseminated granulomatous disease in a cat caused by larvae of Toxocara canis. J Comp Pathol 99:343-6
Badley, J E; Grieve, R B; Rockey, J H et al. (1987) Immune-mediated adherence of eosinophils to Toxocara canis infective larvae: the role of excretory-secretory antigens. Parasite Immunol 9:133-43
Glickman, L T; Shofer, F S (1987) Zoonotic visceral and ocular larva migrans. Vet Clin North Am Small Anim Pract 17:39-53
Bowman, D D; Mika-Grieve, M; Grieve, R B (1987) Circulating excretory-secretory antigen levels and specific antibody responses in mice infected with Toxocara canis. Am J Trop Med Hyg 36:75-82
Badley, J E; Grieve, R B; Bowman, D D et al. (1987) Analysis of Toxocara canis larval excretory-secretory antigens: physicochemical characterization and antibody recognition. J Parasitol 73:593-600

Showing the most recent 10 out of 17 publications