Abstract

Biochemical pathways of visual transduction in frog rod outer segments will be probed using monoclonal antibodies to photoreceptor proteins. (1) Using a number of antibodies to the G-protein already in hand, the functional effects of antibody binding to the G-protein will be characterized in disrupted outer segments by assaying GTP binding, cGMP phosphodiesterase activity, and protein phosphorylation. Antibodies that block G-protein function will be used to study biochemical interrelationships in the light-activated cGMP pathway. (2) Monoclonal antibodies will be generated to other rod outer segment proteins potentially important in visual transduction; e.g. cGMP phosphodiesterase, Components I and II, rhodopsin kinase, cGMP-dependent protein kinase, etc. These antibodies will also be screened for effects on protein function, and then used in studies on the roles of these proteins. (3) Depending on the progress of (1) and (2) G-protein antibodies will be used to probe the structure-function relationships of the G-protein, and its homologies with the G-protein important in many hormone responsive systems.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY006062-02
Application #
3261994
Study Section
Visual Sciences A Study Section (VISA)
Project Start
1985-02-01
Project End
1988-01-31
Budget Start
1986-02-01
Budget End
1988-01-31
Support Year
2
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of Illinois at Chicago
Department
Type
Overall Medical
DUNS #
121911077
City
Chicago
State
IL
Country
United States
Zip Code
60612

  Publications

Kaya, Ali I; Lokits, Alyssa D; Gilbert, James A et al. (2016) A Conserved Hydrophobic Core in G?i1 Regulates G Protein Activation and Release from Activated Receptor. J Biol Chem 291:19674-86
Kaya, Ali I; Iverson, T M; Hamm, Heidi E (2015) Functional stability of rhodopsin in a bicelle system: evaluating G protein activation by rhodopsin in bicelles. Methods Mol Biol 1271:67-76
Kaya, Ali I; Lokits, Alyssa D; Gilbert, James A et al. (2014) A conserved phenylalanine as a relay between the ?5 helix and the GDP binding region of heterotrimeric Gi protein ? subunit. J Biol Chem 289:24475-87
Alexander, Nathan S; Preininger, Anita M; Kaya, Ali I et al. (2014) Energetic analysis of the rhodopsin-G-protein complex links the ?5 helix to GDP release. Nat Struct Mol Biol 21:56-63
Thaker, Tarjani M; Sarwar, Maruf; Preininger, Anita M et al. (2014) A transient interaction between the phosphate binding loop and switch I contributes to the allosteric network between receptor and nucleotide in G?i1. J Biol Chem 289:11331-41
Preininger, Anita M; Meiler, Jens; Hamm, Heidi E (2013) Conformational flexibility and structural dynamics in GPCR-mediated G protein activation: a perspective. J Mol Biol 425:2288-98
Hamm, Heidi E; Kaya, Ali I; Gilbert 3rd, James A et al. (2013) Linking receptor activation to changes in Sw I and II of G* proteins. J Struct Biol 184:63-74
Natarajan, Chandramohan; Hata, Aaron N; Hamm, Heidi E et al. (2013) Extracellular loop II modulates GTP sensitivity of the prostaglandin EP3 receptor. Mol Pharmacol 83:206-16
Thaker, Tarjani M; Kaya, Ali I; Preininger, Anita M et al. (2012) Allosteric mechanisms of G protein-Coupled Receptor signaling: a structural perspective. Methods Mol Biol 796:133-74
Makino, Clint L; Wen, Xiao-Hong; Michaud, Norman A et al. (2012) Rhodopsin expression level affects rod outer segment morphology and photoresponse kinetics. PLoS One 7:e37832

Showing the most recent 10 out of 40 publications