Abstract

Plasma membrane glycoproteins and glycolipids of rabbit and human corneal epithelium in cell culture will be identified and characterized. The role of these glycoconjugates in bacterial adherence and in the pathogenesis of bacterial corneal ulcers will be investigated. Specifically, we will attempt to determine, to which, if any, corneal epithelial cell surface sugars, bacteria adhere and whether it is possible to block such adherence by various lectins, glycosidases, and saccharide moieties. We shall also investigate, which, if any, isolated corneal epithelial cell surface glycoproteins and glycolipids are involved in bacterial adherence. Glycoproteins and glycolipids of migrating and non-migrating rabbit corneal epithelium obtained by organ culture will be identified and characterized. We will investigate, specifically which, if any glycoconjugates are involved in bacterial adherence. Similar preliminary studies will also be performed with migrating and non-migrating epithelium obtained from animals in vivo. Data obtained using the two species and the three techniques will be compared and the validity of the appropriate technique and the selection of the rabbit as a model will, we expect, be established. It is hoped that the proposed investigation will lead to a better understanding of the pathogenesis of bacterial corneal ulcers and of those factors which modulate corneal epithelial cell migration.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY007088-02
Application #
3263982
Study Section
Visual Sciences A Study Section (VISA)
Project Start
1987-08-01
Project End
1990-07-31
Budget Start
1988-08-01
Budget End
1989-07-31
Support Year
2
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
Tufts University
Department
Type
Schools of Medicine
DUNS #
604483045
City
Boston
State
MA
Country
United States
Zip Code
02111

  Publications

Chen, Wei-Sheng; Cao, Zhiyi; Leffler, Hakon et al. (2017) Galectin-3 Inhibition by a Small-Molecule Inhibitor Reduces Both Pathological Corneal Neovascularization and Fibrosis. Invest Ophthalmol Vis Sci 58:9-20
Chen, Wei-Sheng; Cao, Zhiyi; Sugaya, Satoshi et al. (2016) Erratum: Pathological lymphangiogenesis is modulated by galectin-8-dependent crosstalk between podoplanin and integrin-associated VEGFR-3. Nat Commun 7:12063
Sampson, James F; Suryawanshi, Amol; Chen, Wei-Sheng et al. (2016) Galectin-8 promotes regulatory T-cell differentiation by modulating IL-2 and TGF? signaling. Immunol Cell Biol 94:213-9
Chen, Wei-Sheng; Cao, Zhiyi; Sugaya, Satoshi et al. (2016) Pathological lymphangiogenesis is modulated by galectin-8-dependent crosstalk between podoplanin and integrin-associated VEGFR-3. Nat Commun 7:11302
Sampson, James F; Hasegawa, Eiichi; Mulki, Lama et al. (2015) Galectin-8 Ameliorates Murine Autoimmune Ocular Pathology and Promotes a Regulatory T Cell Response. PLoS One 10:e0130772
Chen, Wei-Sheng; Cao, Zhiyi; Truong, Laetitia et al. (2015) Fingerprinting of galectins in normal, P. aeruginosa-infected, and chemically burned mouse corneas. Invest Ophthalmol Vis Sci 56:515-25
Cao, Zhiyi; Saravanan, Chandrassegar; Chen, Wei-Sheng et al. (2015) Examination of the role of galectins in cell migration and re-epithelialization of wounds. Methods Mol Biol 1207:317-26
Sugaya, Satoshi; Chen, Wei-Sheng; Cao, Zhiyi et al. (2015) Comparison of galectin expression signatures in rejected and accepted murine corneal allografts. Cornea 34:675-681
Panjwani, Noorjahan (2014) Role of galectins in re-epithelialization of wounds. Ann Transl Med 2:89
Markowska, Anna I; Cao, Zhiyi; Panjwani, Noorjahan (2014) Glycobiology of ocular angiogenesis. Glycobiology 24:1275-82

Showing the most recent 10 out of 18 publications