The overall goal of the proposed research is to determine the pathway(s) by which visual information is transmitted to the superior colliculus (SC) neurons that innervate nuclei which, in turn, control head and eye movements. The sensory information that deep layer, SC cells send to medullary and spinal structures is thought to be critically involved in orienting and attentional behavior. However, the visual input pathways for these SC neurons have not been defined. Previous physiological and light microscopic experiments have suggested several potential sources of critical visual input to deep layer output cells in the rodent SC. Superficially directed dendrites of deep layer neurons may receive retinal input and/or synapses from the axon collaterals of visually responsive superficial layer cells. Alternatively, direct retinal projections to the deep laminae and/or axonal projections from the superficial to the deep layers might provide the necessary visual input for these cells. We will combine anterograde transport, intracellular horseradish peroxidase injection, and electron microscopic techniques to determine which of these potential pathways actually exist in the hamster's SC. We will then combine electrophysiological recording with reversible inactivation techniques to determine which of the pathways defined in the electron microscope provide(s) the necessary visual input to deep layer output cells.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY008015-03
Application #
3265128
Study Section
Visual Sciences B Study Section (VISB)
Project Start
1988-12-01
Project End
1993-11-30
Budget Start
1990-12-01
Budget End
1991-11-30
Support Year
3
Fiscal Year
1991
Total Cost
Indirect Cost
Name
University of Toledo
Department
Type
Schools of Medicine
DUNS #
807418939
City
Toledo
State
OH
Country
United States
Zip Code
43614
Lane, R D; Allan, D M; Bennett-Clarke, C A et al. (1997) Projection status of calbindin- and parvalbumin-immunoreactive neurons in the superficial layers of the rat's superior colliculus. Vis Neurosci 14:277-86
Lane, R D; Allan, D M; Bennett-Clarke, C A et al. (1996) Differential age-dependent effects of retinal deafferentation upon calbindin- and parvalbumin-immunoreactive neurons in the superficial layers of the rat's superior colliculus. Brain Res 740:208-14
Mooney, R D; Huang, X; Shi, M Y et al. (1996) Serotonin modulates retinotectal and corticotectal convergence in the superior colliculus. Prog Brain Res 112:57-69
Arce, E A; Rhoades, R W; Mooney, R D (1995) Neonatal administration of the neurotoxin 5,7-dihydroxytryptamine results in synaptic reorganization in the superficial gray layer of the hamster's superior colliculus. Vis Neurosci 12:779-83
Arce, E A; Rhoades, R W; Mooney, R D (1995) Effects of neonatal and adult enucleation on the synaptic organization of the serotoninergic projection to the superficial gray layer of the hamster's superior colliculus. Brain Res Dev Brain Res 90:168-73
Arce, E A; Bennett-Clarke, C A; Rhoades, R W (1994) Ultrastructural organization of the noradrenergic innervation of the superficial gray layer of the hamster's superior colliculus. Synapse 18:46-54
Mooney, R D; Zhang, Y; Rhoades, R W (1994) Effects of angiotensin II on visual neurons in the superficial laminae of the hamster's superior colliculus. Vis Neurosci 11:1163-73
Mooney, R D; Shi, M Y; Rhoades, R W (1994) Modulation of retinotectal transmission by presynaptic 5-HT1B receptors in the superior colliculus of the adult hamster. J Neurophysiol 72:3-13
Lane, R D; Bennett-Clarke, C A; Allan, D M et al. (1993) Immunochemical heterogeneity in the tecto-LP pathway of the rat. J Comp Neurol 333:210-22
Huang, X; Mooney, R D; Rhoades, R W (1993) Effects of serotonin on retinotectal-, corticotectal-, and glutamate-induced activity in the superior colliculus of the hamster. J Neurophysiol 70:723-32

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