Abstract

The investigator proposes to isolate cone-specific cDNAs as a means to delineate the function of the visual cascade and cell biology of cone cells in addition to providing candidates for study of the cd dog in specific aim II. To accomplish this, the investigator will use the cd dog, the only available animal model of cone degeneration. Two techniques are proposed to identify mRNA species present in normal dogs, but absent from cd/cd dog retinas. The first is differential display of the two mRNA populations. This will be based on the use of four degenerately tailed oligo dT primers differing only in their last base and 20 10-base upstream primers. Subtractive hybridization will also be used to generate a pool of cone-specific cDNAs, the technique the investigator used to isolate the mouse rd gene.
Specific Aim II. The investigator proposes to identify and characterize the gene lesion responsible for the cd dog disease. To accomplish this, differential display of retina mRNAs from pre-degenerate cd/cd and age matched normal dogs and possibly subtractive hybridization of predegenerate cd/cd retinas with age-matched cd/+ retinal cDNAwill be used. The investigator will use GDRDA if the first two techniques are unsuccessful.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY008285-08
Application #
2654652
Study Section
Special Emphasis Panel (ZRG1-VISB (05))
Project Start
1989-08-01
Project End
2001-01-31
Budget Start
1998-02-01
Budget End
1999-01-31
Support Year
8
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
University of California Los Angeles
Department
Ophthalmology
Type
Schools of Medicine
DUNS #
119132785
City
Los Angeles
State
CA
Country
United States
Zip Code
90095

  Publications

Ahmedli, Novruz B; Gribanova, Yekaterina; Njoku, Collins C et al. (2013) Dynamics of the rhomboid-like protein RHBDD2 expression in mouse retina and involvement of its human ortholog in retinitis pigmentosa. J Biol Chem 288:9742-54
Mokhonov, Vladislav V; Theendakara, Veena P; Gribanova, Yekaterina E et al. (2012) Sequence-specific binding of recombinant Zbed4 to DNA: insights into Zbed4 participation in gene transcription and its association with other proteins. PLoS One 7:e35317
Kitamura, Eiko; Gribanova, Yekaterina E; Farber, Debora B (2011) Regulation of retinoschisin secretion in Weri-Rb1 cells by the F-actin and microtubule cytoskeleton. PLoS One 6:e20707
Saghizadeh, Mehrnoosh; Gribanova, Yekaterina; Akhmedov, Novrouz B et al. (2011) ZBED4, a cone and Muller cell protein in human retina, has a different cellular expression in mouse. Mol Vis 17:2011-8
Saghizadeh, Mehrnoosh; Akhmedov, Novrouz B; Yamashita, Clyde K et al. (2009) ZBED4, a BED-type zinc-finger protein in the cones of the human retina. Invest Ophthalmol Vis Sci 50:3580-8
Saghizadeh, Mehrnoosh; Akhmedov, Novrouz B; Farber, Debora B (2008) Identification and characterization of genes expressed in cone photoreceptors. Adv Exp Med Biol 613:235-44
Yellore, Vivek S; Papp, Jeanette C; Sobel, Eric et al. (2007) Replication and refinement of linkage of posterior polymorphous corneal dystrophy to the posterior polymorphous corneal dystrophy 1 locus on chromosome 20. Genet Med 9:228-34
Kitamura, Eiko; Danciger, Michael; Yamashita, Clyde et al. (2006) Disruption of the gene encoding the beta1-subunit of transducin in the Rd4/+ mouse. Invest Ophthalmol Vis Sci 47:1293-301
Akhmedov, Novrouz B; Yamashita, Clyde K; Tran, Dai et al. (2005) Two forms of the large tumor suppressor gene (Lats1) protein expressed in the vertebrate retina. Biochim Biophys Acta 1728:11-7
Reid, Silvia N M; Yamashita, Clyde; Farber, Debora B (2003) Retinoschisin, a photoreceptor-secreted protein, and its interaction with bipolar and muller cells. J Neurosci 23:6030-40

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