Abstract

The goal of this renewal application is to provide 1) detailed basic cell biology and molecular information about the viral particle that is anterogradely transported; 2) the mechanisms by which HSV is anterograde axonally transported; and 3) the viral gene(s) that are required for anterograde transport. Retinal ganglion cells will be the model, because virus moving in the anterograde direction can be distinguished from that moving in the retrograde direction. The applicants hypothesize that HSV uses normal intracellular targeting mechanisms to accomplish its transport and targeting. With information gained from this proposal, they can begin to unravel the molecular signals involved in the infection and transport of the herpes virus, as well as the mechanisms of infection of other clinical significant neurotropic viruses, such as cytomegalovirus, varicella-zoster virus.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY008773-20
Application #
2711032
Study Section
Neurological Sciences Subcommittee 1 (NLS)
Program Officer
Kerza-Kwiatecki, a P
Project Start
1978-07-01
Project End
2001-06-30
Budget Start
1998-07-01
Budget End
1999-06-30
Support Year
20
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
University of California San Francisco
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143

  Publications

Draper, Jolene M; Stephenson, Graham S; LaVail, Jennifer H (2014) In vivo HSV-1 DNA transport studies using murine retinal ganglion cells. Methods Mol Biol 1144:283-92
LaVail, J H; Tauscher, A N; Sucher, A et al. (2007) Viral regulation of the long distance axonal transport of herpes simplex virus nucleocapsid. Neuroscience 146:974-85
LaVail, Jennifer H; Tauscher, Andrew N; Hicks, James W et al. (2005) Genetic and molecular in vivo analysis of herpes simplex virus assembly in murine visual system neurons. J Virol 79:11142-50
Harrabi, Ons; Tauscher, Andrew N; LaVail, Jennifer H (2004) Temporal expression of herpes simplex virus type 1 mRNA in murine retina. Curr Eye Res 29:191-4
Whitehead, John L; Ohara, Peter T; Tauscher, Andrew N et al. (2003) A procedure to deliver herpes simplex virus to the murine trigeminal ganglion. Brain Res Brain Res Protoc 12:60-6
LaVail, Jennifer H; Tauscher, Andrew N; Aghaian, Elda et al. (2003) Axonal transport and sorting of herpes simplex virus components in a mature mouse visual system. J Virol 77:6117-26
Ohara, P T; Tauscher, A N; LaVail, J H (2001) Two paths for dissemination of Herpes simplex virus from infected trigeminal ganglion to the murine cornea. Brain Res 899:260-3
Ohara, P T; Chin, M S; LaVail, J H (2000) The spread of herpes simplex virus type 1 from trigeminal neurons to the murine cornea: an immunoelectron microscopy study. J Virol 74:4776-86
Bearer, E L; Breakefield, X O; Schuback, D et al. (2000) Retrograde axonal transport of herpes simplex virus: evidence for a single mechanism and a role for tegument. Proc Natl Acad Sci U S A 97:8146-50
Bearer, E L; Schlief, M L; Breakefield, X O et al. (1999) Squid axoplasm supports the retrograde axonal transport of herpes simplex virus. Biol Bull 197:257-8

Showing the most recent 10 out of 22 publications