Two powerful inhibitory networks in the visual thalamus converge on relay cells and influence every spike that travels downstream. Local interneurons provide feedforward inhibition to relay cells and each other. The thalamic reticular nucleus receives input from relay cells and inhibits them in return. Work in fixed tissue or brain slices has provided insight into the pharmacology, cellular physiology and anatomy of these circuits. It is patently necessary apply these ex vivo results to function in vivo. To bridge this gap, we record from inhibitory cells directly and monitor the inhibition they generate in relay cells during vision. Our strategy updates classical comparative anatomical and physiological approaches by combining whole-cell recording and intracellular labeling in vivo with theory and computational techniques.
Aim 1) Exploring the integration of On and Off pathways in the LGN. Relay cells have receptive fields made of concentric On and Off subregions with a push-pull layout of excitation and inhibition;e.g. where bright stimuli excite, dark inhibit. Retina supplies the push (excitation). We propose that the pull (inhibition to stimuli of the reverse sign) comes from local interneurons with receptive fields like those of their postsynaptic partners, but with the opposite preference for stimulus polarity. It is difficult, however, to map connectivity between and On and OFF cells because these cannot be anatomically distinguished in most mammals. Thus, we will test our hypothesis by using the ferret, where On and Off cells occupy different sublaminae in the LGN.
Aim 2) Model systems to explore inhibitory mechanisms in higher animals. Genetic approaches make rodent a popular subject for studying vision. However, the cortical organization of carnivore vs. rodent is vastly different, from the level of the functional architecture to properties of single cells. We ask where these differences emerge by quantitatively comparing the synaptic structure of receptive fields in carnivore vs. rodent LGN. Preliminary studies suggest that basic principles of processing in the LGN are conserved. Thus, we will probe push-pull using mutants lacking an On channel. Further, interneurons and relay cells in cat process their inputs in quantitatively different ways that optimize information transmission;we will dissect the bases for these differences in rodent.
Aim 3) Inhibitory contributions to processing stimulus contrast. We hypothesize that push-pull and same-sign inhibition (inhibition to the preferred stimulus polarity) expand the range of sensitivity to stimulus contrast and improve feature detection at high contrasts. We will explore extra-retinal mechanisms of contrast gain by comparing retinal input to thalamic output patterns in relay cells and by recording from interneurons. Push- pull vs. same-sign inhibition will be separated empirically by silencing the On channel, and computationally with conductance based models. In addition, we will ask how inhibition contributes to feature selectivity by assessing changes in the relative weights of push and pull at different contrasts.

Public Health Relevance

Synapses made by inhibitory neurons dominate the intrinsic circuitry of the visual thalamus and influence all signals traveling from retina to cortex. Here we update classical comparative physiological and anatomical approaches with whole-cell recording in vivo, genetic manipulation and varied computational methods to ask how inhibitory circuits in thalamus enhance selectivity for stimulus features and improve the efficiency of the neural code. Our premise is that without understanding how basic circuits operate in the healthy animal, one cannot appreciate how these circuits are altered by diseases, such as those that strike the eye (amblyopia, strabismus) or cognition (schizophrenia, autism).

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
2R01EY009593-20
Application #
8600828
Study Section
Special Emphasis Panel (ZRG1-IFCN-Q (02))
Program Officer
Steinmetz, Michael A
Project Start
1993-07-01
Project End
2017-07-31
Budget Start
2013-08-01
Budget End
2014-07-31
Support Year
20
Fiscal Year
2013
Total Cost
$410,104
Indirect Cost
$160,104
Name
University of Southern California
Department
None
Type
Schools of Arts and Sciences
DUNS #
072933393
City
Los Angeles
State
CA
Country
United States
Zip Code
90089
Martinez, Luis M; Molano-Mazón, Manuel; Wang, Xin et al. (2014) Statistical wiring of thalamic receptive fields optimizes spatial sampling of the retinal image. Neuron 81:943-56
Koepsell, Kilian; Wang, Xin; Hirsch, Judith A et al. (2010) Exploring the function of neural oscillations in early sensory systems. Front Neurosci 4:53
Wang, Xin; Wei, Yichun; Vaingankar, Vishal et al. (2007) Feedforward excitation and inhibition evoke dual modes of firing in the cat's visual thalamus during naturalistic viewing. Neuron 55:465-78
Martinez, Luis M; Wang, Qingbo; Reid, R Clay et al. (2005) Receptive field structure varies with layer in the primary visual cortex. Nat Neurosci 8:372-9