Abstract

The overall objectives of this research are to identify and characterize the growth factor-receptor systems which regulate corneal development, homeostasis and wound healing.
The specific aims of this application are to (1) study the normal expression of scatter factor (HGF) and fibroblast growth factor-7 (KGF) and their receptors in the developing mouse eye; (2) study transgenic mice engineered to selectively express HGF, HGF dominant-negative receptor, KGF, or KGF dominant-negative receptor in the corneal epithelium; (3) study the effects of cytokines, corticosteroids and wound healing on HGF and KGF expression in the cornea and lacrimal gland and KGF availability in tears; and (4) study the expression and the effect of over-expression of the alternative truncated HGF receptor on corneal epithelial cell function.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY010056-09
Application #
6518497
Study Section
Visual Sciences C Study Section (VISC)
Program Officer
Fisher, Richard S
Project Start
1994-08-01
Project End
2003-08-22
Budget Start
2002-07-01
Budget End
2003-08-22
Support Year
9
Fiscal Year
2002
Total Cost
$359,951
Indirect Cost

  Institution

Name
University of Washington
Department
Ophthalmology
Type
Schools of Medicine
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195

  Publications

Saikia, Paramananda; Thangavadivel, Shanmugapriya; Medeiros, Carla S et al. (2018) IL-1 and TGF-? Modulation of Epithelial Basement Membrane Components Perlecan and Nidogen Production by Corneal Stromal Cells. Invest Ophthalmol Vis Sci 59:5589-5598
Saikia, Paramananda; Medeiros, Carla S; Thangavadivel, Shanmugapriya et al. (2018) Basement membranes in the cornea and other organs that commonly develop fibrosis. Cell Tissue Res 374:439-453
Medeiros, Carla S; Marino, Gustavo K; Lassance, Luciana et al. (2018) The Impact of Photorefractive Keratectomy and Mitomycin C on Corneal Nerves and Their Regeneration. J Refract Surg 34:790-798
Lassance, Luciana; Marino, Gustavo K; Medeiros, Carla S et al. (2018) Fibrocyte migration, differentiation and apoptosis during the corneal wound healing response to injury. Exp Eye Res 170:177-187
Wilson, Steven E; Medeiros, Carla S; Santhiago, Marcony R (2018) Pathophysiology of Corneal Scarring in Persistent Epithelial Defects After PRK and Other Corneal Injuries. J Refract Surg 34:59-64
Medeiros, Carla S; Marino, Gustavo K; Santhiago, Marcony R et al. (2018) The Corneal Basement Membranes and Stromal Fibrosis. Invest Ophthalmol Vis Sci 59:4044-4053
Medeiros, Carla S; Lassance, Luciana; Saikia, Paramananda et al. (2018) Posterior stromal cell apoptosis triggered by mechanical endothelial injury and basement membrane component nidogen-1 production in the cornea. Exp Eye Res 172:30-35
Santhanam, Abirami; Marino, Gustavo K; Torricelli, Andre A M et al. (2017) EBM regeneration and changes in EBM component mRNA expression in stromal cells after corneal injury. Mol Vis 23:39-51
Marino, Gustavo K; Santhiago, Marcony R; Santhanam, Abirami et al. (2017) Epithelial basement membrane injury and regeneration modulates corneal fibrosis after pseudomonas corneal ulcers in rabbits. Exp Eye Res 161:101-105
Wilson, Steven E; Marino, Gustavo K; Torricelli, Andre A M et al. (2017) Injury and defective regeneration of the epithelial basement membrane in corneal fibrosis: A paradigm for fibrosis in other organs? Matrix Biol 64:17-26

Showing the most recent 10 out of 153 publications