Abstract

The purpose of this research is to extend the understanding of the effects of RPE debridement and subsequent RPE cell transplantation on the morphologic changes on the retina and choriocapillaris. Specifically, the applicant will (1) document the geographic extent of choriocapillaris atrophy after debridement of the RPE with and without RPE healing; (2) determine if transplantation of donor RPE immediately after RPE debridement with mitomycin C will prevent atrophy of the choriocapillaris and damage to the overlying retina; (3) determine if delayed RPE transplantation will induce morphologic evidence of regeneration of the choriocapillaris and/or retina; (4) study the fate of RPE cells surgically transplanted onto Bruch's membrane; and (5) determine the mechanism by which transplanted RPE cells attach to Bruch's membrane in vivo.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
2R01EY010311-04
Application #
2019892
Study Section
Visual Sciences C Study Section (VISC)
Project Start
1994-01-01
Project End
2000-12-31
Budget Start
1997-01-01
Budget End
1997-12-31
Support Year
4
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
Washington University
Department
Ophthalmology
Type
Schools of Medicine
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130

  Publications

Del Priore, Lucian V; Tezel, Tongalp H; Kaplan, Henry J (2004) Survival of allogeneic porcine retinal pigment epithelial sheets after subretinal transplantation. Invest Ophthalmol Vis Sci 45:985-92
Del Priore, Lucian V; Kuo, Ya-Hui; Tezel, Tongalp H (2002) Age-related changes in human RPE cell density and apoptosis proportion in situ. Invest Ophthalmol Vis Sci 43:3312-8
Del Priore, Lucian V; Geng, Lee; Tezel, Tongalp H et al. (2002) Extracellular matrix ligands promote RPE attachment to inner Bruch's membrane. Curr Eye Res 25:79-89
Tezel, T H; Kaplan, H J; Del Priore, L V (1999) Fate of human retinal pigment epithelial cells seeded onto layers of human Bruch's membrane. Invest Ophthalmol Vis Sci 40:467-76
Tezel, T H; Del Priore, L V (1999) Repopulation of different layers of host human Bruch's membrane by retinal pigment epithelial cell grafts. Invest Ophthalmol Vis Sci 40:767-74
Tezel, T H; Del Priore, L V (1999) TGF beta secretion modulates the density-dependent growth of pig retinal pigment epithelium in vitro. Ophthalmic Res 31:192-202
Tezel, T H; Del Priore, L V (1998) Serum-free media for culturing and serial-passaging of adult human retinal pigment epithelium. Exp Eye Res 66:807-15
Kaplan, H J; Tezel, T H; Del Priore, L V (1998) Retinal pigment epithelial transplantation in age-related macular degeneration. Retina 18:99-102
Bynoe, L A; Del Priore, L V; Hornbeck, R (1998) Photosensitization of retinal pigment epithelium by protoporphyrin IX. Graefes Arch Clin Exp Ophthalmol 236:230-3
Kim, K S; Tezel, T H; Del Priore, L V (1998) Minimum number of adult human retinal pigment epithelial cells required to establish a confluent monolayer in vitro. Curr Eye Res 17:962-9

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