THIS IS A SHANNON AWARD PROVIDING PARTIAL SUPPORT FOR THE RESEARCH PROJECTS THAT FALL SHORT OF THE ASSIGNED INSTITUTE'S FUNDING RANGE BUT ARE IN THE MARGIN OF EXCELLENCE. THE SHANNON AWARD IS INTENDED TO PROVIDE SUPPORT TO TEST THE FEASIBILITY OF THE APPROACH; DEVELOP FURTHER TESTS AND REFINE RESEARCH TECHNIQUES; PERFORM SECONDARY ANALYSIS OR AVAILABLE DATA SETS; OR CONDUCT DISCRETE PROJECTS THAT CAN DEMONSTRATE THE PI'S RESEARCH CAPABILITIES OR LEND ADDITIONAL WEIGHT TO AN ALREADY MERITORIOUS APPLICATION. THE ABSTRACT BELOW IS TAKEN FROM THE ORIGINAL DOCUMENT SUBMITTED BY THE PRINCIPAL INVESTIGATOR. DESCRIPTION (Adapted from applicant's abstract): The goal of the proposed research is to find genes required for axonal growth, pathfinding and target recognition using a mutagenetic approach. The animal model is the Drosophila optic nerve of the larva which provides a simple model of the aforementioned phenomena. The optic nerve follows a stereotyped path over a connecting bridge of cells and has two regions where growth cones pause and then make directional changes. Using a chemical mutagenesis screen a number of mutants have been isolated that effect the optic nerve at various points along its pathway. The molecular and genetic characterization of one of the mutations called abstract (abs) forms the heart of the proposal. The follow up studies that are proposed will (1) attempt to characterize abs to gain insights into its biochemical function; (2) analyze the phenotype of loss of function or gain of function mutations in the embryo and during imaginal disc formation in hopes of revealing its function; and (3) undertake a genetic analysis of abs as it interacts with other genes to define its role within a more complex background of altered proteins.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY011560-02
Application #
2684579
Study Section
Visual Sciences C Study Section (VISC)
Project Start
1997-04-01
Project End
2002-03-31
Budget Start
1998-04-01
Budget End
1999-03-31
Support Year
2
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Rockefeller University
Department
Genetics
Type
Other Domestic Higher Education
DUNS #
071037113
City
New York
State
NY
Country
United States
Zip Code
10065
Kurant, Estee; Axelrod, Sofia; Leaman, Dan et al. (2008) Six-microns-under acts upstream of Draper in the glial phagocytosis of apoptotic neurons. Cell 133:498-509
Bainton, Roland J; Tsai, Linus T-Y; Schwabe, Tina et al. (2005) moody encodes two GPCRs that regulate cocaine behaviors and blood-brain barrier permeability in Drosophila. Cell 123:145-56
Schwabe, Tina; Bainton, Roland J; Fetter, Richard D et al. (2005) GPCR signaling is required for blood-brain barrier formation in drosophila. Cell 123:133-44