Abstract

New technologies have allowed us to image the immune response in vivo in 3 spatial dimensions as well as time. The rodent eye is ideal for in vivo imaging of the immune response. The eye is a well recognized target of immune-mediated inflammation known as uveitis. We have succeeded in imaging leukocyte-endothelial interactions in the inflamed mouse and rat eye in a system that involves no surgical trauma and which allows repetitive observation over many days. Using the technique of ocular intravital microscopy, we propose to: 1) determine the role of antigen specific T cells in immune-mediated or autoimmune inflammation; 2) determine the role of specific adhesion molecules or cytokines in an in vivo model of immune-mediated inflammation; 3) identify the site and kinetics of T cell activation in vivo in a disease model; and 4) enhance the technology that we are currently using to obtain 4 dimensional imaging of the immune response. These studies offer a unique opportunity to develop modalities to image the immune response in vivo.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY013093-03
Application #
6384895
Study Section
Special Emphasis Panel (ZAI1-ALR-I (S1))
Program Officer
Liberman, Ellen S
Project Start
1999-09-30
Project End
2004-09-29
Budget Start
2001-09-30
Budget End
2002-09-29
Support Year
3
Fiscal Year
2001
Total Cost
$528,500
Indirect Cost

  Institution

Name
Oregon Health and Science University
Department
Ophthalmology
Type
Schools of Medicine
DUNS #
009584210
City
Portland
State
OR
Country
United States
Zip Code
97239

  Publications

Willermain, François; Rosenbaum, James T; Bodaghi, Bahram et al. (2012) Interplay between innate and adaptive immunity in the development of non-infectious uveitis. Prog Retin Eye Res 31:182-94
Zhang, Zili; Wu, Xiumei; Duan, Jie et al. (2012) Low dose rapamycin exacerbates autoimmune experimental uveitis. PLoS One 7:e36589
Wu, Xiumei; Rosenbaum, James T; Adamus, Grazyna et al. (2011) Activation of OX40 prolongs and exacerbates autoimmune experimental uveitis. Invest Ophthalmol Vis Sci 52:8520-6
Zhang, Zili; Rosenbaum, James T; Zhong, Wenwei et al. (2010) Costimulation of Th17 cells: Adding fuel or putting out the fire in the inflamed gut? Semin Immunopathol 32:55-70
Myronenko, Andriy; Song, Xubo (2010) Intensity-based image registration by minimizing residual complexity. IEEE Trans Med Imaging 29:1882-91
Rosenzweig, Holly L; Jann, Monica J; Vance, Emily E et al. (2010) Nucleotide-binding oligomerization domain 2 and Toll-like receptor 2 function independently in a murine model of arthritis triggered by intraarticular peptidoglycan. Arthritis Rheum 62:1051-9
Zhang, Zili; Zhong, Wenwei; Hinrichs, David et al. (2010) Activation of OX40 augments Th17 cytokine expression and antigen-specific uveitis. Am J Pathol 177:2912-20
Lee, Ellen J; Rosenbaum, James T; Planck, Stephen R (2010) Epifluorescence intravital microscopy of murine corneal dendritic cells. Invest Ophthalmol Vis Sci 51:2101-8
Zhong, Wenwei; Zhang, Zili; Hinrichs, David et al. (2010) OX40 induces CCL20 expression in the context of antigen stimulation: an expanding role of co-stimulatory molecules in chemotaxis. Cytokine 50:253-9
Zhang, Zili; Zhong, Wenwei; Spencer, Doran et al. (2009) Interleukin-17 causes neutrophil mediated inflammation in ovalbumin-induced uveitis in DO11.10 mice. Cytokine 46:79-91

Showing the most recent 10 out of 31 publications