Age-related macular degeneration (AMD) is a highly prevalent, late-onset complex disorder caused by a combination of interacting genetic and environmental factors. Recent studies have identified several Mendelian loci, most notably those encoding proteins of the complement system, which are highly associated with the development of AMD. These loci currently explain between 50-70% of the disease, thus other causal factors most certainly participate in the disease development. Indeed, multiple lines of evidence suggest a role for various environmental factors in AMD. It has been proposed, for example, that diet, smoking, and photochemical damage increase oxidative stress, thereby contributing to development of AMD. These environmental factors act through non-Mendelian genetic mechanisms, including epigenetic regulation of gene expression and damage to mitochondrial DNA (mtDNA), which have been documented in various late-onset complex traits. None of these disease mechanisms has been comprehensively studied in AMD. The major goal of this proposal is to test the hypothesis that the non-Mendelian genetic mechanisms such as copy number variation of genes, epigenetic regulation of gene expression and somatic variation in mtDNA play a significant role in AMD. To investigate this hypothesis, we will utilize a unique repository comprised of 4,000 extensively characterized (both clinically and genetically) human donor eyes and advanced methods of genetic analyses to address the following specific aims: 1) refinement of our knowledge relating to AMD endo- phenotypes and genotype/phenotype correlation by enhanced phenotyping of AMD cases using advanced image analysis;2) determination of specific genes/loci where copy number variation is associated with AMD;3) assessment of epigenetic regulation of gene expression in the development of AMD and;4) assessment of the role of acquired, somatic variation in mtDNA in susceptibility to AMD. The discovery of additional genetic and "environmental" causes of AMD will contribute important new information pertaining to disease etiology, and will reveal potential new surrogate biomarkers and diagnostic tests. More importantly, the proposed project will likely provide for new pharmacological targets for the treatment of this extremely prevalent and devastating disorder.
AMD is the most prevalent cause of vision loss in the elderly, affecting millions of Americans representing a major public health problem with currently limited treatment options that are palliative in nature. Several major Mendelian genetic loci associated with AMD (explaining roughly 50% of the disorder) have been identified, however, less is known about the other half possibly consisting of environmental and non-Mendelian components. Identification of copy number variation of genes, their methylation/expression status and variation in mitochondrial DNA and the resulting pathophysiological effects will contribute new information pertaining to disease etiology, will reveal potential surrogate biomarkers and diagnostic tests, and will suggest pharmacological targets for the treatment of this extremely prevalent and devastating disorder.
|Grassmann, Felix; Friedrich, Ulrike; Fauser, Sascha et al. (2015) A Candidate Gene Association Study Identifies DAPL1 as a Female-Specific Susceptibility Locus for Age-Related Macular Degeneration (AMD). Neuromolecular Med 17:111-20|
|Schubert, Carl; Pryds, Anders; Zeng, Shemin et al. (2014) Cadherin 5 is regulated by corticosteroids and associated with central serous chorioretinopathy. Hum Mutat 35:859-67|
|Ratnapriya, Rinki; Zhan, Xiaowei; Fariss, Robert N et al. (2014) Rare and common variants in extracellular matrix gene Fibrillin 2 (FBN2) are associated with macular degeneration. Hum Mol Genet 23:5827-37|
|Garg, Aakriti; Wapner, Ronald J; Ananth, Cande V et al. (2014) Choroidal and retinal thickening in severe preeclampsia. Invest Ophthalmol Vis Sci 55:5723-9|
|Fritsche, Lars G; Chen, Wei; Schu, Matthew et al. (2013) Seven new loci associated with age-related macular degeneration. Nat Genet 45:433-9, 439e1-2|
|Burke, Tomas R; Yzer, Suzanne; Zernant, Jana et al. (2013) Abnormality in the external limiting membrane in early Stargardt disease. Ophthalmic Genet 34:75-7|
|Sobrin, Lucia; Ripke, Stephan; Yu, Yi et al. (2012) Heritability and genome-wide association study to assess genetic differences between advanced age-related macular degeneration subtypes. Ophthalmology 119:1874-85|
|Chen, Royce W S; Greenberg, Jonathan P; Lazow, Margot A et al. (2012) Autofluorescence imaging and spectral-domain optical coherence tomography in incomplete congenital stationary night blindness and comparison with retinitis pigmentosa. Am J Ophthalmol 153:143-54.e2|
|Burke, Tomas R; Tsang, Stephen H; Zernant, Jana et al. (2012) Familial discordance in Stargardt disease. Mol Vis 18:227-33|
|Baas, Dominique C; Ho, Lintje; Tanck, Michael W T et al. (2012) Multicenter cohort association study of SLC2A1 single nucleotide polymorphisms and age-related macular degeneration. Mol Vis 18:657-74|
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