The retina detects and transmits large amounts of visual information quickly and reliably. Ribbon synapses are key components of the vertebrate retinal circuitry, forming the first and second presynaptic elements in the signaling pathway to the brain. The specialized morphology and function of the ribbons presumably endows them with a unique capacity for copious and fast neurotransmitter release, which is thought to be essential for the efficient processing and encoding of visual information. Nevertheless, the underlying cellular mechanisms that modulate and maintain transmitter output from ribbon synapses under vastly different ambient light conditions are poorly understood. Due to their large size, we are able to patch-clamp single goldfish bipolar cell terminals. This allows us to measure both presynaptic Ca currents and evoked changes in membrane capacitance that assay synaptic vesicle exocytosis and endocytosis in real time from a living nerve terminal. We have found that the reciprocal synapse of bipolar cells undergoes short-term synaptic depression. We will determine the underlying mechanisms responsible for this depression. The first hypothesis to be tested is that synaptic vesicle pool depletion at GABAergic amacrine cells, and desensitization of GABAA and AMPA receptors, contribute to depression and largely determine the recovery rate. We also find that bipolar cell terminals have a tonic inhibitory current mediated by high affinity GABAC receptors that do not desensitize. Thus the second hypothesis is that different subtypes GABA transporters in amacrine cells set the level of this tonic inhibitory current. We also have preliminary evidence that the acidity of synaptic vesicles and the process of filling synaptic vesicles with glutamate may involve chloride channels on the vesicle membrane. Biochemical studies of this process are controversial and few studies have been done in intact nerve terminals. We will thus study this process in the bipolar cell terminal embedded in a retinal slice. These studies should increase our basic understanding of signal processing in the retina and they may be relevant to retinal diseases that degenerate photoreceptors, but spare ganglion cells. A prosthetic device that stimulates the remaining neurons might restore some sight to blind people. However, this stimulation has to encode visual information at the same high rates that bipolar cells do in normal retinas. A better understanding of how bipolar cells release glutamate to excite ganglion cells will thus lead to insights on how to develop a retinal prosthesis using more physiologically relevant patterns of stimulation that match more closely the original information rates of bipolar cells. Our studies will thus hopefully aid in the future design of more efficient retinal prosthesis devices.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY014043-09
Application #
7822727
Study Section
Biology and Diseases of the Posterior Eye Study Section (BDPE)
Program Officer
Greenwell, Thomas
Project Start
2002-05-01
Project End
2012-04-30
Budget Start
2010-05-01
Budget End
2011-04-30
Support Year
9
Fiscal Year
2010
Total Cost
$343,035
Indirect Cost
Name
Oregon Health and Science University
Department
Neurosciences
Type
Schools of Medicine
DUNS #
096997515
City
Portland
State
OR
Country
United States
Zip Code
97239
Kim, Mean-Hwan; von Gersdorff, Henrique (2016) Postsynaptic Plasticity Triggered by Ca²?-Permeable AMPA Receptor Activation in Retinal Amacrine Cells. Neuron 89:507-20
Taxidis, Jiannis; Anastassiou, Costas A; Diba, Kamran et al. (2015) Local Field Potentials Encode Place Cell Ensemble Activation during Hippocampal Sharp Wave Ripples. Neuron 87:590-604
Balakrishnan, Veeramuthu; Puthussery, Theresa; Kim, Mean-Hwan et al. (2015) Synaptic Vesicle Exocytosis at the Dendritic Lobules of an Inhibitory Interneuron in the Mammalian Retina. Neuron 87:563-75
Kim, Mean-Hwan; Li, Geng-Lin; von Gersdorff, Henrique (2013) Single Ca2+ channels and exocytosis at sensory synapses. J Physiol 591:3167-78
Vickers, Evan; Kim, Mean-Hwan; Vigh, Jozsef et al. (2012) Paired-pulse plasticity in the strength and latency of light-evoked lateral inhibition to retinal bipolar cell terminals. J Neurosci 32:11688-99
Cho, Soyoun; von Gersdorff, Henrique (2012) Ca(2+) influx and neurotransmitter release at ribbon synapses. Cell Calcium 52:208-16
Vigh, Jozsef; Vickers, Evan; von Gersdorff, Henrique (2011) Light-evoked lateral GABAergic inhibition at single bipolar cell synaptic terminals is driven by distinct retinal microcircuits. J Neurosci 31:15884-93
Calero, Cecilia I; Vickers, Evan; Moraga Cid, Gustavo et al. (2011) Allosteric modulation of retinal GABA receptors by ascorbic acid. J Neurosci 31:9672-82
Kim, Mean-Hwan; von Gersdorff, Henrique (2010) Extending the realm of membrane capacitance measurements to nerve terminals with complex morphologies. J Physiol 588:2011-2
Yamashita, Takayuki; Eguchi, Kohgaku; Saitoh, Naoto et al. (2010) Developmental shift to a mechanism of synaptic vesicle endocytosis requiring nanodomain Ca2+. Nat Neurosci 13:838-44

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