The broad, long-term objective of the proposed research is the laboratory demonstration and clinical introduction of a noninvasive ophthalmologic imaging technology to characterize changes in the neurotubules of retinal ganglion cell (RGC) axons associated with the onset and progression of glaucoma. Although numerous damage mechanisms and apoptotic signaling pathways are under investigation in glaucoma research, the pathologic hallmark for glaucoma in human eyes is selective death of RGCs and accompanying degeneration of axonal neurotubules within the retinal nerve fiber layer (RNFL). Enhanced polarization-sensitive optical coherence tomography (EPS-OCT) combines the high resolution cross-sectional imaging capability of optical coherence tomography (OCT) with the polarimetric-sensitivity of scanning laser polarimetry (SLP) to simultaneously measure RNFL thickness (ZRNFL), retardation (SRNFL and birefringence (AnRNFL) with high sensitivity and accuracy. Because RNFL birefringence (AnKNFL) is directly related to the density of neurotubules in RGC axons, the principal investigator and colleagues believe research studies proposed herein will establish that EPS-OCT can objectively and noninvasively monitor RGC neurotubule density (o>GC) and therefore has significant advantages in sensitivity and specificity over existing methods for diagnosis and monitoring of glaucoma.
The specific aims of this proposal are to: 1. Construct a fiber-based spectral domain EPS-OCT instrument for measuring RNFL birefringence (AnRHFL) and verify that the instrument has sensitivity and accuracy necessary for noninvasive quantification of RGC neurotubule density (o>cc) in the RNFL. 2. Experimentally validate the mathematical relationship between RNFL birefringence (AnRNFL) and RGC neurotubule density (o>cc) using the fiber-based spectral domain EPS-OCT instrument and transmission electron microscope (TEM) histology. 3. Characterize the temporal and spatial dynamics of RNFL birefringence (^nRNFL) and RGC neurotubule density (crflcc) during the progression from healthy to advanced glaucoma in experimental rat and primate glaucoma models. 4. Using ROC analysis, formulate and test a feature set and classifier based on RNFL thickness (ZKNFL), retardation (SRNFL), and birefringence (AnRNFL) for discriminating between normal and glaucomatous human eyes.

National Institute of Health (NIH)
National Eye Institute (NEI)
Research Project (R01)
Project #
Application #
Study Section
Special Emphasis Panel (ZRG1-BDCN-F (12))
Program Officer
Agarwal, Neeraj
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Texas Austin
Biomedical Engineering
Schools of Engineering
United States
Zip Code
Yin, Biwei; Dwelle, Jordan; Wang, Bingqing et al. (2015) Fourier optics analysis of phase-mask-based path-length-multiplexed optical coherence tomography. J Opt Soc Am A Opt Image Sci Vis 32:2169-77
Liu, Shuang; Datta, Anjali; Ho, Derek et al. (2015) Effect of image registration on longitudinal analysis of retinal nerve fiber layer thickness of non-human primates using Optical Coherence Tomography (OCT). Eye Vis (Lond) 2:3
Liu, Shuang; Wang, Bingqing; Yin, Biwei et al. (2014) Retinal nerve fiber layer reflectance for early glaucoma diagnosis. J Glaucoma 23:e45-52
Yin, Biwei; Wang, Bingqing; Rylander 3rd, Henry G et al. (2014) Degradation in the degree of polarization in human retinal nerve fiber layer. J Biomed Opt 19:16001
Yin, Biwei; Kuranov, Roman V; McElroy, Austin B et al. (2013) Dual-wavelength photothermal optical coherence tomography for imaging microvasculature blood oxygen saturation. J Biomed Opt 18:56005
Wang, Bingqing; Yin, Biwei; Dwelle, Jordan et al. (2013) Path-length-multiplexed scattering-angle-diverse optical coherence tomography for retinal imaging. Opt Lett 38:4374-7
Dwelle, Jordan; Liu, Shuang; Wang, Bingqing et al. (2012) Thickness, phase retardation, birefringence, and reflectance of the retinal nerve fiber layer in normal and glaucomatous non-human primates. Invest Ophthalmol Vis Sci 53:4380-95
Elmaanaoui, Badr; Wang, Bingqing; Dwelle, Jordan C et al. (2011) Birefringence measurement of the retinal nerve fiber layer by swept source polarization sensitive optical coherence tomography. Opt Express 19:10252-68
Paranjape, Amit S; Kuranov, Roman; Baranov, Stepan et al. (2010) Depth resolved photothermal OCT detection of macrophages in tissue using nanorose. Biomed Opt Express 1:2-16
Villard, Joseph W; Paranjape, Amit S; Victor, Danielle A et al. (2009) Applications of optical coherence tomography in cardiovascular medicine, Part 2. J Nucl Cardiol 16:620-39

Showing the most recent 10 out of 13 publications