Age-related macular degeneration (AMD) is a disease of the retinal pigment epithelium characterized by the appearance of protein/lipid deposits (drusen), geographic atrophy and neovascularization. Techniques currently used for diagnostic imaging of AMD include indocyanine green dye or fluorescein angiography, autofluorescence imaging and optical coherence tomography (OCT). OCT images depict optical backscatter, caused by variation in local optical refractive index. The high resolution provided by OCT has proved invaluable for imaging structural changes associated with AMD. Nevertheless, our understanding of the pathobiology of this disease is uncertain. The development of high-resolution retinal/choroidal imaging modalities not dependent upon optical scattering offers a potential means for gaining new insights into the AMD disease process, early diagnosis and clinical management. In this project we propose to develop and test means for imaging the elastic properties of the retina/choroid by detection of minute displacements generated within these structures induced by acoustic radiation force. Such elastic changes at the level of Bruch's membrane and the choroid would be expected as a consequence of drusen deposition and altered microvascular patterns. Furthermore, tissue elastic changes are also known to be associated with the development of neovascularization. A capacity to image elastic alteration may provide a means to visualize precursors to pathologic changes associated with AMD disease progression. We propose to image the retina/choroid with high speed, high-resolution, enhanced-depth OCT and to observe minute displacements occurring upon exposure to acoustic radiation force at safe, diagnostic levels. We will utilize two techniques: acoustic radiation force impulse (ARFI) imaging, in which displacements are induced by a brief (order of 1 msec) impulse, and vibro-acoustography, in which displacements are induced by low-frequency (order of 1 kHz) vibrations induced by interaction of two ultrasound sources of slightly different frequency. Studies will be conducted with tissue phantoms, rabbit eyes, in a primate model of AMD and finally normal and AMD human subjects in which we will demonstrate alterations in retinal/choroidal elastic properties associated with specific pathologic conditions including drusen, geographic atrophy, and neovascularization.
The aim of this project is improved imaging and early diagnosis of age-related macular degeneration (AMD), a major cause of blindness. Current imaging techniques such as optical coherence tomography (OCT) allow detailed display of retinal anatomy, but cannot probe tissue elastic properties which may be altered as part of the disease process. We will utilize OCT to image the response of the retina and choroid to compressional pulses or vibrations induced by ultrasound in animal models and subjects with AMD to image retinal/choroidal elasticity and relate this property to the presence of drusen, disease progression and response to treatment.
|Qi, Li; Zhu, Jiang; Hancock, Aneeka M et al. (2016) Fully distributed absolute blood flow velocity measurement for middle cerebral arteries using Doppler optical coherence tomography. Biomed Opt Express 7:601-15|
|Piao, Zhonglie; Zeng, Lvming; Chen, Zhongping et al. (2016) Q-switched Erbium-doped fiber laser at 1600â€‰nm for photoacoustic imaging application. Appl Phys Lett 108:143701|
|Qu, Yueqiao; Ma, Teng; He, Youmin et al. (2016) Acoustic Radiation Force Optical Coherence Elastography of Corneal Tissue. IEEE J Sel Top Quantum Electron 22:|
|Xu, Xiangqun; Zhu, Jiang; Chen, Zhongping (2016) Dynamic and quantitative assessment of blood coagulation using optical coherence elastography. Sci Rep 6:24294|
|Zhu, Jiang; Qi, Li; Miao, Yusi et al. (2016) 3D mapping of elastic modulus using shear wave optical micro-elastography. Sci Rep 6:35499|
|Li, Jiawen; Minami, Hataka; Steward, Earl et al. (2015) Optimal flushing agents for integrated optical and acoustic imaging systems. J Biomed Opt 20:56005|
|Heidari, Andrew E; Moghaddam, Samer; Truong, Kimberly K et al. (2015) Visualizing biofilm formation in endotracheal tubes using endoscopic three-dimensional optical coherence tomography. J Biomed Opt 20:126010|
|Li, Jiawen; Ma, Teng; Mohar, Dilbahar et al. (2015) Ultrafast optical-ultrasonic system and miniaturized catheter for imaging and characterizing atherosclerotic plaques in vivo. Sci Rep 5:18406|
|Piao, Zhonglie; Ma, Teng; Li, Jiawen et al. (2015) High speed intravascular photoacoustic imaging with fast optical parametric oscillator laser at 1.7â€‰Î¼m. Appl Phys Lett 107:083701|
|Qi, Li; Huang, Shenghai; Heidari, Andrew E et al. (2015) Automatic airway wall segmentation and thickness measurement for long-range optical coherence tomography images. Opt Express 23:33992-4006|
Showing the most recent 10 out of 27 publications