Neural circuits of the primary visual cortex are critical for generating accurate perceptions of our external world. These circuits are functionally organized across both the vertical and horizontal axes of the visual cortex. Nonetheless, the distinct contributions of vertical and horizontal projections to visual computation are poorly understood. In particular, the balance of excitation and inhibition (the E/I balance) that vertical and horizontal projections generate during sensory evoked activity is not well characterized due to a lack of appropriate methodology. The first goal of this proposal is to implement a new approach to selectively activate the vertical and horizontal projections for each layer of the cortex using a combination of cell-type specific gene targeting and optogenetic tools. The second goal is to use this new approach to test the hypothesis that vertical and horizontal projections generate layer-specific E/I balances in the cortex that determine their functional impact on cortical activity. The third goal is to establish how the distinct E/I balances that vertical and horizontal projections generate in visual cortical circuits mediate the encoding of fundamental stimulus features such as contrast and size.

Public Health Relevance

The balance of excitation and inhibition in neural circuits is a critical parameter that determines how activity propagates in the brain. Disruptions of this balance in the cerebral cortex can lead to a variety of neurological disorders. Thus understanding how specific neural circuits in the cortex contribute to the balance of excitation and inhibition will provide critical insight to understand the neural basis of perception and identify the underlying causes of neurological diseases.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
1R01EY023756-01
Application #
8562737
Study Section
Neurotransporters, Receptors, and Calcium Signaling Study Section (NTRC)
Program Officer
Steinmetz, Michael A
Project Start
2013-09-01
Project End
2018-08-31
Budget Start
2013-09-01
Budget End
2014-08-31
Support Year
1
Fiscal Year
2013
Total Cost
$382,583
Indirect Cost
$132,583
Name
University of California Berkeley
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
124726725
City
Berkeley
State
CA
Country
United States
Zip Code
94704