The GOAL of this research is to establish a scientific basis for cost effective prevention of conjunctivitis. Bacterial conjunctivitis is a costly diseaseof children. There are >4 million office visits in the US/yr. Among the most common infections of childhood, it is a leading cause for antibiotic use. Half of cases occur in children under 9 years, with an annual cost of over $500 million. Bacterial conjunctivitis is caused by unusual strains of unencapsulated S. pneumoniae that are largely incapable of causing disease at other body sites and are uniquely pathogenic for the eye. Falling between specialties of Ophthalmology and Infectious Diseases, these unusual microbes have largely gone unstudied. An especially large and well documented conjunctivitis outbreak, involving 698 students at Dartmouth College, and six months later, the same strain caused another outbreak among 101 young children and 120 family contacts in Maine. Vaccines target the primary virulence factor of S. pneumoniae - the capsule. However, the vaccine is completely ineffective in preventing conjunctivitis, a disease uniquely caused by strains that lack the capsule, leaving an unaddressed gap in coverage. If we understood the basis for the unusual tropism of unencapsulated pneumococci for the ocular surface - that is, the key factors allowing it to bind and colonize the eye - we could include epitopes of these factors in the vaccine already being used, thereby preventing a common disease of childhood, and saving hundreds of $ million in health care costs, and limiting a leading cause for treating children with antibiotics, which is now believed to be altering their flra as well as leading to antibiotic resistance. In preliminary studies, we undertook an expansive comparative genomics study to identify candidate factors that allow unencapsulated S. pneumoniae to bind to the ocular surface. This research proposes to follow up these leads by directly testing a prioritized list of the most strongly implicated adhesins and other colonization factors, for their ability to mediate attachment to the ocular surface. The proposed studies combine biological approaches, as well as state-of-the-art genomics approaches, to identify the most important colonization factors that lead to conjunctivitis.

Public Health Relevance

Bacterial conjunctivitis, a common disease of children, adds over $ 500 million/yr to health care costs and is a leading cause for childhood use of antibiotics. A leading cause for this disease, S.pneumoniae, is completely missed by the vaccine because of the unusual properties of these eye-specific strains. This work aims to identify properties that could make the existing vaccine effective for prevent ocular infections as well.

National Institute of Health (NIH)
National Eye Institute (NEI)
Research Project (R01)
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Mckie, George Ann
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Massachusetts Eye and Ear Infirmary
United States
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Van Tyne, Daria; Ciolino, Joseph B; Wang, Jay et al. (2016) Novel Phagocytosis-Resistant Extended-Spectrum ?-Lactamase-Producing Escherichia coli From Keratitis. JAMA Ophthalmol 134:1306-1309
Shainheit, Mara G; Valentino, Michael D; Gilmore, Michael S et al. (2015) Mutations in pneumococcal cpsE generated via in vitro serial passaging reveal a potential mechanism of reduced encapsulation utilized by a conjunctival isolate. J Bacteriol 197:1781-91
Valentino, Michael D; McGuire, Abigail Manson; Rosch, Jason W et al. (2014) Unencapsulated Streptococcus pneumoniae from conjunctivitis encode variant traits and belong to a distinct phylogenetic cluster. Nat Commun 5:5411