In order to obtain time-resolved resonance Raman spectra and fluorescence spectra of biological molecules, we found it necessary to replace the current SIT detector with a more sensitive detector such as PAR model 1420 intensified silicon photodiode array detector which is capable of high speed gating - 5 nsec with high on/off ratio (10 to the 6th powr:1). This detector should enable us to reject fluorescence with left-time 5 nsec or longer. We plan to use this new multichannel system to investigate oxy-coboglobin and spinach nitrate reductase.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM018894-16
Application #
3269442
Study Section
Biophysics and Biophysical Chemistry B Study Section (BBCB)
Project Start
1978-09-01
Project End
1987-08-31
Budget Start
1986-09-01
Budget End
1987-08-31
Support Year
16
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Georgia Institute of Technology
Department
Type
Schools of Arts and Sciences
DUNS #
097394084
City
Atlanta
State
GA
Country
United States
Zip Code
30332
Chiou, S H; Lee, B S; Yu, N T (1992) Structural analysis and comparison of cobrotoxin and cardiotoxins by near-IR Fourier transform Raman spectroscopy. Biochem Int 26:747-58
Hori, H; Tsubaki, M; Yu, N T et al. (1991) Light absorption, electron paramagnetic resonance and resonance Raman characteristics of nitridochromium(V) protoporphyrin-IX and its reconstituted hemoproteins. Biochim Biophys Acta 1077:392-9
Chiou, S H; Lee, B S; Chang, C C et al. (1991) Structural analysis of phospholipase A2 by near-IR Fourier transform Raman spectroscopy. Biochem Int 25:387-95
Lin, S H; Yu, N T; Tame, J et al. (1990) Effect of the distal residues on the vibrational modes of the Fe-CO bond in hemoglobin studied by protein engineering. Biochemistry 29:5562-6
Liu, H H; Lin, S H; Yu, N T (1990) Resonance Raman enhancement of phenyl ring vibrational modes in phenyl iron complex of myoglobin. Biophys J 57:851-6
Nie, S M; Bergbauer, K L; Kuck Jr, J F et al. (1990) Near-infrared Fourier transform Raman spectroscopy in human lens research. Exp Eye Res 51:619-23
Yu, N T; Lin, S H; Chang, C K et al. (1989) Resonance Raman enhancement of the Mn-N-O bending mode in nitrosyl manganese ""strapped"" and ""open"" heme complexes. Biophys J 55:1137-44
Gersonde, K; Yu, N T; Lin, S H et al. (1989) Resonance Raman assignment and evidence for noncoupling of individual 2- and 4-vinyl vibrational modes in a monomeric cyanomethemoglobin. Biochemistry 28:3960-6
Lin, S H; Yu, N T; Gersonde, K (1988) Resonance Raman evidence for an unusually strong exogenous ligand-metal bond in a monomeric nitrosyl manganese hemoglobin. FEBS Lett 229:367-71
Thompson, H M; Yu, N T; Gersonde, K (1987) Resonance Raman evidence for the mechanism of the allosteric control of O2-binding in a cobalt-substituted monomeric insect hemoglobin. Biophys J 51:289-95

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