Abstract

Osmotic adaptation is a fundamental problem in cell biology. Recent work in our own and other laboratories has provided strong evidence for the important role of membrane-derived oligosaccharides (MDO) of E. coli in osmotic adaptation and cell signalling, and for the functions of the closely similar periplasmic cyclic glucans of the Rhizobiaceae. This application is focussed on the MDO of E. coli with the following specific goals. I. THE BIOSYNTHESIS OF MDO A. The Function of ACP B. The Function of Polyprenol Derivatives C. Search for Protein-bound Intermediates II. GENETIC REGULATION OF MDO BIOSYNTHESIS A. Enzyme Activities and mdo Phenotypes B. Search for in vitro Complementation C. Mapping of mdo Mutations III. MECHANISM OF OSMOTIC REGULATION OF MOD SYNTHESIS A. Time Course of Osmotic Adaptation B. Osmotic Regulation of Enzyme Levels C. Feedback Inhibition of MDO Synthesis

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM022057-18
Application #
3270888
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1975-05-01
Project End
1993-04-30
Budget Start
1992-05-01
Budget End
1993-04-30
Support Year
18
Fiscal Year
1992
Total Cost
Indirect Cost

  Institution

Name
Harvard University
Department
Type
Schools of Medicine
DUNS #
082359691
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Tang, L; Weissborn, A C; Kennedy, E P (1997) Domains of Escherichia coli acyl carrier protein important for membrane-derived-oligosaccharide biosynthesis. J Bacteriol 179:3697-705
Weissborn, A C; Liu, Q; Rumley, M K et al. (1994) UTP: alpha-D-glucose-1-phosphate uridylyltransferase of Escherichia coli: isolation and DNA sequence of the galU gene and purification of the enzyme. J Bacteriol 176:2611-8
Weissborn, A C; Rumley, M K; Kennedy, E P (1992) Isolation and characterization of Escherichia coli mutants blocked in production of membrane-derived oligosaccharides. J Bacteriol 174:4856-9
Geiger, O; Russo, F D; Silhavy, T J et al. (1992) Membrane-derived oligosaccharides affect porin osmoregulation only in media of low ionic strength. J Bacteriol 174:1410-3
Spaink, H P; Aarts, A; Stacey, G et al. (1992) Detection and separation of Rhizobium and Bradyrhizobium Nod metabolites using thin-layer chromatography. Mol Plant Microbe Interact 5:72-80
Rumley, M K; Therisod, H; Weissborn, A C et al. (1992) Mechanisms of regulation of the biosynthesis of membrane-derived oligosaccharides in Escherichia coli. J Biol Chem 267:11806-10
Geiger, O; Spaink, H P; Kennedy, E P (1991) Isolation of the Rhizobium leguminosarum NodF nodulation protein: NodF carries a 4'-phosphopantetheine prosthetic group. J Bacteriol 173:2872-8
Weissborn, A C; Rumley, M K; Kennedy, E P (1991) Biosynthesis of membrane-derived oligosaccharides. Membrane-bound glucosyltransferase system from Escherichia coli requires polyprenyl phosphate. J Biol Chem 266:8062-7
Geiger, O; Weissborn, A C; Kennedy, E P (1991) Biosynthesis and excretion of cyclic glucans by Rhizobium meliloti 1021. J Bacteriol 173:3021-4
Fischl, A S; Kennedy, E P (1990) Isolation and properties of acyl carrier protein phosphodiesterase of Escherichia coli. J Bacteriol 172:5445-9

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