Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM022131-19
Application #
2173875
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1978-05-01
Project End
1998-06-30
Budget Start
1996-07-01
Budget End
1998-06-30
Support Year
19
Fiscal Year
1996
Total Cost
Indirect Cost
Name
Purdue University
Department
Biochemistry
Type
Schools of Earth Sciences/Natur
DUNS #
072051394
City
West Lafayette
State
IN
Country
United States
Zip Code
47907
Wang, Y; Zhao, S; Somerville, R L et al. (2001) Solution structure of the DNA-binding domain of the TyrR protein of Haemophilus influenzae. Protein Sci 10:592-8
Kristl, S; Zhao, S; Knappe, B et al. (2000) The influence of ATP on the binding of aromatic amino acids to the ligand response domain of the tyrosine repressor of Haemophilus influenzae. FEBS Lett 467:87-90
Zhao, S; Zhu, Q; Somerville, R L (2000) The sigma(70) transcription factor TyrR has zinc-stimulated phosphatase activity that is inhibited by ATP and tyrosine. J Bacteriol 182:1053-61
Zhao, S; Somerville, R L (1999) Isolated operator binding and ligand response domains of the TyrR protein of Haemophilus influenzae associate to reconstitute functional repressor. J Biol Chem 274:1842-7
Smith, H Q; Somerville, R L (1997) The tpl promoter of Citrobacter freundii is activated by the TyrR protein. J Bacteriol 179:5914-21
Zhu, Q; Zhao, S; Somerville, R L (1997) Expression, purification, and functional analysis of the TyrR protein of Haemophilus influenzae. Protein Expr Purif 10:237-46
Chen, Y W; Dekker, E E; Somerville, R L (1995) Functional analysis of E. coli threonine dehydrogenase by means of mutant isolation and characterization. Biochim Biophys Acta 1253:208-14
Zhao, G P; Somerville, R L; Chitnis, P R (1994) Synechocystis PCC 6803 contains a single gene for the beta subunit of tryptophan synthase with strong homology to the trpB genes of Arabidopsis and maize (Zea mays L.). Plant Physiol 104:461-6
Cui, J; Somerville, R L (1993) The TyrR protein of Escherichia coli, analysis by limited proteolysis of domain structure and ligand-mediated conformational changes. J Biol Chem 268:5040-7
Cui, J; Somerville, R L (1993) Mutational uncoupling of the transcriptional activation function of the TyrR protein of Escherichia coli K-12 from the repression function. J Bacteriol 175:303-6

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