Inflammatory response, tissue repair and regeneration, cancer, arthritis, atherosclerosis, and congenital brain defects. Understanding cell migration is challenging, since it requires understanding both the mechanisms that drive and regulate its component processes, e.g., front-back polarization, protrusion, and the formation and disassembly of adhesions, and the mechanisms that integrated them spatially and temporally. This renewal builds on the significant progress that we have made over the past 4 years;they include new imaging modalities for mapping molecular interactions and dynamics at high spatial resolution, identification of a new class of adhesions, nascent adhesions, that drive migration by signaling to Rac near the leading edge, demonstration that myosin II polarizes the cell and regulates and spatially integrates the component processes that comprise cell migration, visualization of adhesions in 3-dimensional matrices, and the demonstration that dendritic spine dynamics and post synaptic density organization are highly analogous to adhesion and protrusion in migrating cells. In this renewal, we develop these insights and bring state of the art imaging methods for identifying protein complexes to understand the nucleation and early events in adhesion assembly, determine when, where, and how adhesions generate migration-related signals and what regulates them, determine how myosin II generates front-back polarity and contact inhibition of migration, and how myosin II mediates mechanotransduction by interpreting and integrating extracellular pliability. We translate these observations to migration in 3-dimensions and to dendritic spine morphology and post synaptic density organization in hippocampal neurons. We will study neuronal and fibroblastic cells using a prioritized list of signaling and structural molecules. The proposed studies bear directly on tumor cell invasion, chronic inflammation, mental retardation, schizophrenia, and regenerative therapies using stem cell transplantation.

Public Health Relevance

Our immediate goal is to understand the machinery that drives cell migration, its regulation, and adaptation to different the chemical and physical environments that cells encounter. We then want to translate this understanding to develop diagnostic and therapeutic strategies for the many processes and diseases that are migration-related, which include tumor invasion and metastasis, chronic inflammatory diseases and nonsyndromic mental retardation, as well as regenerative strategies for wound repair and cellular transplantation to recreate functional tissues and organs.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM023244-36A1
Application #
8185802
Study Section
Intercellular Interactions (ICI)
Program Officer
Flicker, Paula F
Project Start
1988-01-01
Project End
2015-06-30
Budget Start
2011-09-26
Budget End
2012-06-30
Support Year
36
Fiscal Year
2011
Total Cost
$613,933
Indirect Cost
Name
University of Virginia
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
065391526
City
Charlottesville
State
VA
Country
United States
Zip Code
22904
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Li, Wei; Mukherjee, Abir; Wu, Jinhua et al. (2015) Sperm Associated Antigen 6 (SPAG6) Regulates Fibroblast Cell Growth, Morphology, Migration and Ciliogenesis. Sci Rep 5:16506

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