Inflammatory response, tissue repair and regeneration, cancer, arthritis, atherosclerosis, and congenital brain defects. Understanding cell migration is challenging, since it requires understanding both the mechanisms that drive and regulate its component processes, e.g., front-back polarization, protrusion, and the formation and disassembly of adhesions, and the mechanisms that integrated them spatially and temporally. This renewal builds on the significant progress that we have made over the past 4 years;they include new imaging modalities for mapping molecular interactions and dynamics at high spatial resolution, identification of a new class of adhesions, nascent adhesions, that drive migration by signaling to Rac near the leading edge, demonstration that myosin II polarizes the cell and regulates and spatially integrates the component processes that comprise cell migration, visualization of adhesions in 3-dimensional matrices, and the demonstration that dendritic spine dynamics and post synaptic density organization are highly analogous to adhesion and protrusion in migrating cells. In this renewal, we develop these insights and bring state of the art imaging methods for identifying protein complexes to understand the nucleation and early events in adhesion assembly, determine when, where, and how adhesions generate migration-related signals and what regulates them, determine how myosin II generates front-back polarity and contact inhibition of migration, and how myosin II mediates mechanotransduction by interpreting and integrating extracellular pliability. We translate these observations to migration in 3-dimensions and to dendritic spine morphology and post synaptic density organization in hippocampal neurons. We will study neuronal and fibroblastic cells using a prioritized list of signaling and structural molecules. The proposed studies bear directly on tumor cell invasion, chronic inflammation, mental retardation, schizophrenia, and regenerative therapies using stem cell transplantation.
Our immediate goal is to understand the machinery that drives cell migration, its regulation, and adaptation to different the chemical and physical environments that cells encounter. We then want to translate this understanding to develop diagnostic and therapeutic strategies for the many processes and diseases that are migration-related, which include tumor invasion and metastasis, chronic inflammatory diseases and nonsyndromic mental retardation, as well as regenerative strategies for wound repair and cellular transplantation to recreate functional tissues and organs.
|Bachir, Alexia I; Horwitz, Alan Rick; Nelson, W James et al. (2017) Actin-Based Adhesion Modules Mediate Cell Interactions with the Extracellular Matrix and Neighboring Cells. Cold Spring Harb Perspect Biol 9:|
|Martin-Vilchez, Samuel; Whitmore, Leanna; Asmussen, Hannelore et al. (2017) RhoGTPase Regulators Orchestrate Distinct Stages of Synaptic Development. PLoS One 12:e0170464|
|Kubow, Kristopher E; Shuklis, Victoria D; Sales, Dominic J et al. (2017) Contact guidance persists under myosin inhibition due to the local alignment of adhesions and individual protrusions. Sci Rep 7:14380|
|Cross, A M; Wilson, A L; Guerrero, M S et al. (2016) Breast cancer antiestrogen resistance 3-p130(Cas) interactions promote adhesion disassembly and invasion in breast cancer cells. Oncogene 35:5850-5859|
|Ramos, Grasieli de Oliveira; Bernardi, Lisiane; Lauxen, Isabel et al. (2016) Fibronectin Modulates Cell Adhesion and Signaling to Promote Single Cell Migration of Highly Invasive Oral Squamous Cell Carcinoma. PLoS One 11:e0151338|
|Horwitz, Rick (2016) Integrated, multi-scale, spatial-temporal cell biology--A next step in the post genomic era. Methods 96:3-5|
|Devreotes, Peter; Horwitz, Alan Rick (2015) Signaling networks that regulate cell migration. Cold Spring Harb Perspect Biol 7:a005959|
|Newell-Litwa, Karen A; Badoual, Mathilde; Asmussen, Hannelore et al. (2015) ROCK1 and 2 differentially regulate actomyosin organization to drive cell and synaptic polarity. J Cell Biol 210:225-42|
|Newell-Litwa, Karen A; Horwitz, Rick; Lamers, Marcelo L (2015) Non-muscle myosin II in disease: mechanisms and therapeutic opportunities. Dis Model Mech 8:1495-515|
|Juanes-Garcia, Alba; Chapman, Jessica R; Aguilar-Cuenca, Rocio et al. (2015) A regulatory motif in nonmuscle myosin II-B regulates its role in migratory front-back polarity. J Cell Biol 209:23-32|
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