The overall aim is to understand how the environment of an enzyme active site controls the reactivity of a catalytic center containing two metal ions connected by a bridging ligand. Bridged bimetallic centers appear in hundreds of different enzymes catalyzing dozens of different reactions including the degradation and synthesis of DNA, RNA and phospholipids; hydride ion transfer; phosphoryl group transfer; the hydrolysis of sugars; and the covalent modification and N-terminal processing of polypeptides. Yet why two metal ions are employed, and how they cooperate in catalysis is not understood.
The specific aims focus on: i) how the metal ions cooperate, ii) why particular metal/ligand combinations are most effective in catalyzing particular types of reactions, and iii) how the rest of the active site participates in, and helps to direct the chemistry of, catalysis. Four representative enzymes have been selected for this study. In xylose isomerase a bridged Mg2+-Mg2+ center converts glucose to fructose, a reaction of enormous commercial importance. Aminopeptidase uses a Zn2+-Zn2+ center in an exopeptidase reaction; members of this enzyme superfamily are targets for antiangiogenic cancer drugs. DRAG uses Mg2+-Mg2+ to catalyze the hydrolysis of ADP-ribose from a covalently-modified protein. And the immunosuppressant target calcineurin uses an Fe3+-Zn2+ center in its role as an essential protein Ser/Thr phosphatase in signal transduction. Our experimental plan is guided by the hypothesis that bridged bimetallic enzymes use the metal ion Lewis acidities in their binuclear sites to: i) bind and position substrate, ii) bind and activate a water molecule to yield an active site hydroxide nucleophile, and iii) act as a """"""""superelectrophile"""""""" to polarize a chemical bond on the substrate and thereby promote formation of the transition state of the catalytic reaction. We will employ a variety of methods, including ones we have developed ourselves. These techniques include protein crystallography (conventional, ultra-high resolution, and time-resolved), neutron Laue crystallography, enzyme kinetics, spectroscopy (in collaboration), quantum mechanics/molecular mechanics calculations, and site-directed mutagenesis.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM026788-23
Application #
6882618
Study Section
Physical Biochemistry Study Section (PB)
Program Officer
Flicker, Paula F
Project Start
1990-04-01
Project End
2006-07-14
Budget Start
2005-04-01
Budget End
2006-07-14
Support Year
23
Fiscal Year
2005
Total Cost
$310,491
Indirect Cost
Name
Brandeis University
Department
Type
Organized Research Units
DUNS #
616845814
City
Waltham
State
MA
Country
United States
Zip Code
02454
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