Abstract

This proposal addresses several questions about the cytoskeleton in dividing cells and nerve cells and about the cell cycle. Using a variety of methods, including frog eggs and extracts derived from them and nerve cells in tissue culture, these experiments will investigate a wide range of issues. There are six specific aims.
In aim 1, a factor from Xenopus embryo extracts that increases the depolymerization of microtubules will be purified and its gene cloned and characterized.
In aim 2 the role of the actin cytoskeleton in the surface contraction waves seen in Xenopus eggs will be examined.
In aim 3 mechanisms of microtubule transport will be examined in culture hippocampal neuron axons and dendrites.
In aim 4 the interaction between actin and the microtubule cytoskeleton will be investigated in neuronal growth cones.
In aim 5 the regulation of G1 cyclins will be examined, particularly defining the targets of cyclin E/cdk2 needed for the activation of DNA replication.
In aim 6 the activation of cdc2 by cyclin B and the identity and role of other activators and inhibitors will be studied.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM026875-20
Application #
2021818
Study Section
Molecular Cytology Study Section (CTY)
Project Start
1978-12-01
Project End
1999-11-30
Budget Start
1996-12-01
Budget End
1997-11-30
Support Year
20
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
Harvard University
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
082359691
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Ginzberg, Miriam Bracha; Chang, Nancy; D'Souza, Heather et al. (2018) Cell size sensing in animal cells coordinates anabolic growth rates and cell cycle progression to maintain cell size uniformity. Elife 7:
Liu, Shixuan; Ginzberg, Miriam Bracha; Patel, Nish et al. (2018) Size uniformity of animal cells is actively maintained by a p38 MAPK-dependent regulation of G1-length. Elife 7:
Lee, Ho-Joon; Jedrychowski, Mark P; Vinayagam, Arunachalam et al. (2017) Proteomic and Metabolomic Characterization of a Mammalian Cellular Transition from Quiescence to Proliferation. Cell Rep 20:721-736
Lu, Ying; Wu, Jiayi; Dong, Yuanchen et al. (2017) Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle. Mol Cell 67:322-333.e6
Chen, Shuobing; Wu, Jiayi; Lu, Ying et al. (2016) Structural basis for dynamic regulation of the human 26S proteasome. Proc Natl Acad Sci U S A 113:12991-12996
Brown, Nicholas G; VanderLinden, Ryan; Watson, Edmond R et al. (2016) Dual RING E3 Architectures Regulate Multiubiquitination and Ubiquitin Chain Elongation by APC/C. Cell 165:1440-1453
Lee, Byung-Hoon; Lu, Ying; Prado, Miguel A et al. (2016) USP14 deubiquitinates proteasome-bound substrates that are ubiquitinated at multiple sites. Nature 532:398-401
Ginzberg, Miriam B; Kafri, Ran; Kirschner, Marc (2015) Cell biology. On being the right (cell) size. Science 348:1245075
Klein, Allon M; Mazutis, Linas; Akartuna, Ilke et al. (2015) Droplet barcoding for single-cell transcriptomics applied to embryonic stem cells. Cell 161:1187-1201
Wang, Weiping; Wu, Tao; Kirschner, Marc W (2014) The master cell cycle regulator APC-Cdc20 regulates ciliary length and disassembly of the primary cilium. Elife 3:e03083

Showing the most recent 10 out of 76 publications