The application is the first competitive review of the PI's MERIT award, during which much progress was made on gene silencing in Saccharomyces cerevisise. During this period, the genetic approaches that have been a strength of the lab have been complemented with new expertise in protein biochemistry, protein structure, genomics and computation. The three big goals of this research are: to understand how RNAi-independent gene silencing occurs leading to the formation of heterochromatin, how specialized structures of chromatin achieve their epigenetic inheritance, and how nutrition can affect epigenetic processes. On the mechanism of silencing, the PI proposes experiments to resolve the contradictory data from the Gross and Widom labs regarding how Sir proteins limit transcription factors'functions in heterochromatin. The NAD metabolite 2-0- Acetyl ADP Ribose, the product of the Sir2 histone deacetylase has gained much interest from in vitro suggestions for a role in silencing. The PI proposes three possible roles for the metabolite and critical in vivo tests of each role. Work on comparative silencing in related species has identified a Sir3 backup mechanism and provide a context that will allow testing of the growth versus oozing models for how heterochromatin spreads. Computational analysis of silencer sequences revealed a Rap1 binding site that is more conserved between species than are Rap1 binding sites within the same species. A hypothesis for this unprecedented conservation and a compelling test are proposed. With respect to the inheritance of the silenced state, a model is presented involving the segregation of Sir protein complexes on histone H3-H4 tetramers that makes specific quantitative predictions about how the stability of these silenced states should vary with the length of the silenced domain. An elegant test of the hypothesis is described that measures rates of change in heritable transcription states. In the recent past, the PI has uncovered links between H2A.Z acetylation, boundary function and the Bdf1 and Yta7 bromodomain proteins, which motivate experiments to uncover how chromatin boundaries function. Isotype-specific modifications and functions of the two H2Bs of yeast will be explored. Finally, the PI has uncovered a fascinating link between nutrition, in the context of folic acid, and heritable gene silencing through histone hypomethylation, whose mechanistic basis will be tested.

Public Health Relevance

The inheritance of stable states of gene expression is relevant to the orderly development of all plants and animals and to the propagation of disease states such as cancer. Moreover, the link between nutritional status and epigenetic states is particularly important because 9% of people have two defective copies of a gene that, based upon the results here, are likely to impact heritable features on their chromosomes.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM031105-31
Application #
8292194
Study Section
Molecular Genetics C Study Section (MGC)
Program Officer
Carter, Anthony D
Project Start
1982-07-01
Project End
2013-06-30
Budget Start
2012-07-01
Budget End
2013-06-30
Support Year
31
Fiscal Year
2012
Total Cost
$538,490
Indirect Cost
$175,028
Name
University of California Berkeley
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
124726725
City
Berkeley
State
CA
Country
United States
Zip Code
94704
Ellahi, Aisha; Rine, Jasper (2016) Evolution and Functional Trajectory of Sir1 in Gene Silencing. Mol Cell Biol 36:1164-79
McCleary, David F; Steakley, David Lee; Rine, Jasper (2016) Sir protein-independent repair of dicentric chromosomes in Saccharomyces cerevisiae. Mol Biol Cell 27:2879-83
Dodson, Anne E; Rine, Jasper (2016) Donor Preference Meets Heterochromatin: Moonlighting Activities of a Recombinational Enhancer in Saccharomyces cerevisiae. Genetics :
Liu, Tzu-Yu; Dodson, Anne E; Terhorst, Jonathan et al. (2016) Riches of phenotype computationally extracted from microbial colonies. Proc Natl Acad Sci U S A 113:E2822-31
Lombardi, Laura M; Davis, Matthew D; Rine, Jasper (2015) Maintenance of nucleosomal balance in cis by conserved AAA-ATPase Yta7. Genetics 199:105-16
Ellahi, Aisha; Thurtle, Deborah M; Rine, Jasper (2015) The Chromatin and Transcriptional Landscape of Native Saccharomyces cerevisiae Telomeres and Subtelomeric Domains. Genetics 200:505-21
Dodson, Anne E; Rine, Jasper (2015) Heritable capture of heterochromatin dynamics in Saccharomyces cerevisiae. Elife 4:e05007
Janke, Ryan; Dodson, Anne E; Rine, Jasper (2015) Metabolism and epigenetics. Annu Rev Cell Dev Biol 31:473-96
Steakley, David Lee; Rine, Jasper (2015) On the Mechanism of Gene Silencing in Saccharomyces cerevisiae. G3 (Bethesda) 5:1751-63
Rine, Jasper; Fagen, Adam P (2015) The State of Federal Research Funding in Genetics as Reflected by Members of the Genetics Society of America. Genetics 200:1015-9

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