Differential gene expression and the spatial distribution of differentially synthesized proteins within a cell lies at the core of the developmental process in both prokaryotes and eukaryotes. To ultimately define the regulatory circuitry which controls these processes, I believe it is essential to understand the structural and regulatory genes responsible for clearly defined morphogenetic events and to study the expression of these genes within the context of other cellular functions. The objectives of this research proposal are to understand the mechanisms by which the Caulobacter polar flagellum is assembled at a specific site on the cell and at a specific time in the cell cycle. To do this we will analyze the differentially synthesized protein components of the flagellum and initiate a program to study the differentially expressed chemotaxis sensory transduction system which controls flagellar rotation. A selected sample of the multiple genes involved in flagellum biogenesis and function will be isolated, mapped, and analyzed with particular attention to genomic arrangement and the function of regulatory sequences. Those, and flagellar regulatory and structural genes already isolated, will be used to determine the steps in the Caulobacter differentiation process which are controlled at the transcriptional and/or post-transcriptional level.
| Mann, Thomas H; Shapiro, Lucy (2018) Integration of cell cycle signals by multi-PAS domain kinases. Proc Natl Acad Sci U S A 115:E7166-E7173 |
| Perez, Adam M; Mann, Thomas H; Lasker, Keren et al. (2017) A Localized Complex of Two Protein Oligomers Controls the Orientation of Cell Polarity. MBio 8: |
| Schrader, Jared M; Li, Gene-Wei; Childers, W Seth et al. (2016) Dynamic translation regulation in Caulobacter cell cycle control. Proc Natl Acad Sci U S A 113:E6859-E6867 |
| Lasker, Keren; Schrader, Jared M; Men, Yifei et al. (2016) CauloBrowser: A systems biology resource for Caulobacter crescentus. Nucleic Acids Res 44:D640-5 |
| Ricci, Dante P; Melfi, Michael D; Lasker, Keren et al. (2016) Cell cycle progression in Caulobacter requires a nucleoid-associated protein with high AT sequence recognition. Proc Natl Acad Sci U S A 113:E5952-E5961 |
| Mann, Thomas H; Seth Childers, W; Blair, Jimmy A et al. (2016) A cell cycle kinase with tandem sensory PAS domains integrates cell fate cues. Nat Commun 7:11454 |
| Lasker, Keren; Mann, Thomas H; Shapiro, Lucy (2016) An intracellular compass spatially coordinates cell cycle modules in Caulobacter crescentus. Curr Opin Microbiol 33:131-139 |
| Schrader, Jared M; Shapiro, Lucy (2015) Synchronization of Caulobacter crescentus for investigation of the bacterial cell cycle. J Vis Exp : |
| Zhou, Bo; Schrader, Jared M; Kalogeraki, Virginia S et al. (2015) The global regulatory architecture of transcription during the Caulobacter cell cycle. PLoS Genet 11:e1004831 |
| Ptacin, Jerod L; Gahlmann, Andreas; Bowman, Grant R et al. (2014) Bacterial scaffold directs pole-specific centromere segregation. Proc Natl Acad Sci U S A 111:E2046-55 |
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