Abstract

The goal of the proposed work is to understand at the molecular level the transport of molecules into and out of the eukaryotic cell nucleus. This work is divided into two areas of investigation: 1) A study of the transport and assembly of the small nuclear RNA (snRNA) U1 and its associated proteins. For this study we will determine which portions of the RNA molecule are necessary for assembly into RNP particles and which portions are needed for transport of the particles into the nucleus. We will ask, using modified U1 RNA molecules, whether transport through the nuclear pores can be sterically blocked. 2) In the second part of the proposed work, we will ask what features of the nucleus itself are required for transport of the model U1 RNP particle. This will be done using the new finding that nuclei can be reconstituted from any type of DNA when that DNA is injected into Xenopus eggs. Intermediates in the formation of nuclei can be used to identify when binding and transport of nuclear proteins occurs. We will also ask: a) whether there are developmental differences in nuclear transport in the early Xenopus embryo, b) whether nuclear transport is energy-driven, and c) whether transport of snRNP particles into nuclei can occur in vitro.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM033279-03
Application #
3282768
Study Section
Molecular Biology Study Section (MBY)
Project Start
1984-04-01
Project End
1987-03-31
Budget Start
1986-04-01
Budget End
1987-03-31
Support Year
3
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of California San Diego
Department
Type
Schools of Arts and Sciences
DUNS #
077758407
City
La Jolla
State
CA
Country
United States
Zip Code
92093

  Publications

Tam, Arvin B; Roberts, Lindsay S; Chandra, Vivek et al. (2018) The UPR Activator ATF6 Responds to Proteotoxic and Lipotoxic Stress by Distinct Mechanisms. Dev Cell 46:327-343.e7
Forbes, Douglass J; Travesa, Anna; Nord, Matthew S et al. (2015) Nuclear transport factors: global regulation of mitosis. Curr Opin Cell Biol 35:78-90
Schwartz, Michal; Travesa, Anna; Martell, Steven W et al. (2015) Analysis of the initiation of nuclear pore assembly by ectopically targeting nucleoporins to chromatin. Nucleus 6:40-54
Torres-Machorro, Ana Lilia; Clark, Lauren G; Chang, Christie S et al. (2015) The Set3 Complex Antagonizes the MYST Acetyltransferase Esa1 in the DNA Damage Response. Mol Cell Biol 35:3714-25
Bernis, Cyril; Forbes, Douglass J (2014) Analysis of nuclear reconstitution, nuclear envelope assembly, and nuclear pore assembly using Xenopus in vitro assays. Methods Cell Biol 122:165-91
Bernis, Cyril; Swift-Taylor, Beth; Nord, Matthew et al. (2014) Transportin acts to regulate mitotic assembly events by target binding rather than Ran sequestration. Mol Biol Cell 25:992-1009
Basnet, Harihar; Su, Xue B; Tan, Yuliang et al. (2014) Tyrosine phosphorylation of histone H2A by CK2 regulates transcriptional elongation. Nature 516:267-71
Eustice, Moriah; Pillus, Lorraine (2014) Unexpected function of the glucanosyltransferase Gas1 in the DNA damage response linked to histone H3 acetyltransferases in Saccharomyces cerevisiae. Genetics 196:1029-39
Powers, Maureen A; Forbes, Douglass J (2012) Nuclear transport: beginning to gel? Curr Biol 22:R1006-9
Fichtman, Boris; Ramos, Corinne; Rasala, Beth et al. (2010) Inner/Outer nuclear membrane fusion in nuclear pore assembly: biochemical demonstration and molecular analysis. Mol Biol Cell 21:4197-211

Showing the most recent 10 out of 45 publications