Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM035533-11
Application #
2177950
Study Section
Pharmacology A Study Section (PHRA)
Project Start
1985-07-01
Project End
1998-07-31
Budget Start
1996-08-01
Budget End
1997-07-31
Support Year
11
Fiscal Year
1996
Total Cost
Indirect Cost
Name
University of California San Francisco
Department
Anesthesiology
Type
Schools of Medicine
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143
Yang, Yuting; Bu, Weishu; Im, Sangchoul et al. (2018) Structure of cytochrome P450 2B4 with an acetate ligand and an active site hydrogen bond network similar to oxyferrous P450cam. J Inorg Biochem 185:17-25
Xia, Chuanwu; Rwere, Freeborn; Im, Sangchoul et al. (2018) Structural and Kinetic Studies of Asp632 Mutants and Fully Reduced NADPH-Cytochrome P450 Oxidoreductase Define the Role of Asp632 Loop Dynamics in the Control of NADPH Binding and Hydride Transfer. Biochemistry 57:945-962
Rwere, Freeborn; Xia, Chuanwu; Im, Sangchoul et al. (2016) Mutants of Cytochrome P450 Reductase Lacking Either Gly-141 or Gly-143 Destabilize Its FMN Semiquinone. J Biol Chem 291:14639-61
Yamamoto, Kazutoshi; Caporini, Marc A; Im, Sang-Choul et al. (2015) Cellular solid-state NMR investigation of a membrane protein using dynamic nuclear polarization. Biochim Biophys Acta 1848:342-9
Zhang, Meng; Le Clair, Stéphanie V; Huang, Rui et al. (2015) Insights into the role of substrates on the interaction between cytochrome b5 and cytochrome P450 2B4 by NMR. Sci Rep 5:8392
Zhang, Meng; Huang, Rui; Im, Sang-Choul et al. (2015) Effects of membrane mimetics on cytochrome P450-cytochrome b5 interactions characterized by NMR spectroscopy. J Biol Chem 290:12705-18
Yang, Yuting; Zhang, Haoming; Usharani, Dandamudi et al. (2014) Structural and functional characterization of a cytochrome P450 2B4 F429H mutant with an axial thiolate-histidine hydrogen bond. Biochemistry 53:5080-91
Vivekanandan, Subramanian; Ahuja, Shivani; Im, Sang-Choul et al. (2014) ¹H, ¹³C and ¹?N resonance assignments for the full-length mammalian cytochrome b? in a membrane environment. Biomol NMR Assign 8:409-13
Yamamoto, Kazutoshi; Gildenberg, Melissa; Ahuja, Shivani et al. (2013) Probing the transmembrane structure and topology of microsomal cytochrome-p450 by solid-state NMR on temperature-resistant bicelles. Sci Rep 3:2556
Hagen, Katharine D; Gillan, James M; Im, Sang-Choul et al. (2013) Electrochemistry of mammalian cytochrome P450 2B4 indicates tunable thermodynamic parameters in surfactant films. J Inorg Biochem 129:30-4

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