These investigations will focus on using molecular biological, biochemical, and genetic methods to elucidate how messenger RNA is degraded in bacteria. Particular attention will be devoted to RppH, a recently discovered RNA pyrophosphohydrolase that triggers the degradation of primary transcripts in E. coli by a previously unrecognized mechanism: the rate-determining conversion of the 5'-terminal triphosphate to a monophosphate. First, the features of RNAs that determine their susceptibility to decay by this mechanism will be identified. In addition, the characteristics of RppH that are important for its activity and the cellular factors that may influence such 5'-end- dependent RNA degradation will be examined. Finally, the contribution of this pathway to mRNA decay in other bacterial species will be explored. The results of these studies will provide important insights into a fundamental aspect of gene regulation that presently is poorly understood. This knowledge should be of value in clarifying a biological regulatory mechanism that can play a key role in bacterial pathogenesis.

Public Health Relevance

The proposed research will address the mechanism of bacterial messenger RNA degradation, a basic biological process important for controlling gene expression in all living organisms. The knowledge thereby acquired should be of value in maximizing bacterial production of medically useful proteins and in elucidating a regulatory mechanism that can influence bacterial pathogenesis.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
3R01GM035769-26S1
Application #
8669324
Study Section
Program Officer
Bender, Michael T
Project Start
2013-09-01
Project End
2015-08-31
Budget Start
2013-09-01
Budget End
2015-08-31
Support Year
26
Fiscal Year
2013
Total Cost
$80,004
Indirect Cost
$32,804
Name
New York University
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
121911077
City
New York
State
NY
Country
United States
Zip Code
10016
Luciano, Daniel J; Vasilyev, Nikita; Richards, Jamie et al. (2018) Importance of a diphosphorylated intermediate for RppH-dependent RNA degradation. RNA Biol 15:703-706
Luciano, Daniel J; Belasco, Joel G (2018) Analysis of RNA 5' ends: Phosphate enumeration and cap characterization. Methods :
Gao, Ang; Vasilyev, Nikita; Luciano, Daniel J et al. (2018) Structural and kinetic insights into stimulation of RppH-dependent RNA degradation by the metabolic enzyme DapF. Nucleic Acids Res 46:6841-6856
Belasco, Joel G (2017) Death by translation: ribosome-assisted degradation of mRNA by endonuclease toxins. FEBS Lett 591:1851-1852
Bischler, Thorsten; Hsieh, Ping-Kun; Resch, Marcus et al. (2017) Identification of the RNA Pyrophosphohydrolase RppH of Helicobacter pylori and Global Analysis of Its RNA Targets. J Biol Chem 292:1934-1950
Lodato, Patricia B; Thuraisamy, Thujitha; Richards, Jamie et al. (2017) Effect of RNase E deficiency on translocon protein synthesis in an RNase E-inducible strain of enterohemorrhagic Escherichia coli O157:H7. FEMS Microbiol Lett 364:
Luciano, Daniel J; Vasilyev, Nikita; Richards, Jamie et al. (2017) A Novel RNA Phosphorylation State Enables 5' End-Dependent Degradation in Escherichia coli. Mol Cell 67:44-54.e6
Richards, Jamie; Belasco, Joel G (2016) Distinct Requirements for 5'-Monophosphate-assisted RNA Cleavage by Escherichia coli RNase E and RNase G. J Biol Chem 291:5038-48
Luciano, Daniel J; Belasco, Joel G (2015) NAD in RNA: unconventional headgear. Trends Biochem Sci 40:245-7
Foley, Patricia L; Hsieh, Ping-kun; Luciano, Daniel J et al. (2015) Specificity and evolutionary conservation of the Escherichia coli RNA pyrophosphohydrolase RppH. J Biol Chem 290:9478-86

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