In this proposal we apply the novel mechanistic oral absorption strategy we have developed to the important category of polar antiviral drugs. Progress during the previous grant period has demonstrated that the transport and activation strategy can be remarkable successful. Further, we have identified the novel activating enzyme, Valacyclovirase and determined the X-ray structure of this novel enzyme that activates valacyclovir and valganciclovir. Most significantly, the X-ray structure and our studies to date with nucleoside prodrugs indicates that this enzyme has a broad leaving group pocket (drug side), indicating that VACVase maybe a versatile prodrug activating enzyme. In this proposal we focus on demonstrating that this mechanistic oral absorption strategy can improve the oral absorption of the very important antiviral drugs, zanamivir and guanidino oseltamivir carboxylate. We will develop a specific substrate and analogues to fully explore the specificty and enzymology of VACVase as a prodrug target. We show very exciting preliminary results that the prodrugs of zanamivir and a highly potent oseltamivir carboxylate analogue have more than 10 fold higher epithelial cell permeability than the parent drug and further that these prodrugs are activated by VACVase.
The specific aims of this proposal are: 1. Synthesize a range of prodrugs of the antiviral drugs, zanamivir and guanidino oseltamivir carboxylate and the specific substrate, Val-HPG, varying the pro-group and the linker and evaluate both chemical and enzymatic stability. 2. Determine the mucosal transport and activation of the prodrugs, evaluate the uptake in HeLa cells, transport permeability and activation in Caco-2 monolayers, and in vivo in the in situ perfused rat jejunum. Select at least 4 prodrugs for in vivo plasma level determination and bioavailability and pharmacokinetic analysis. 3. Determine valacyclovirase hydrolysis and its potential utility as a oral prodrug activating target through kinetics studies of specific substrate analogues for VACVase and determine the structure activity relationship (SAR) of valacyclovirase (VACVase) with the antiviral prodrugs and the specific substrate analogues. In summary, we will develop and extend a mechanistic strategy for improving oral bioavailability through membrane transporter and activating enzyme targeting and apply this strategy to important antiviral drugs. Further, the mechanistic strategy developed in this proposal can be applied to significantly increase the range of therapeutic agents that can be delivered via the oral route of administration.

Public Health Relevance

Enhancing the oral absorption of drug candidates will greatly increase the number and efficacy of drugs available to effectively treat disease. Many potential drugs cannot be used because of poor oral absorption. This project will develop a strategy based on current influenza drugs that is mechanistically based on oral transport and activation in the intestinal tract. This will enable the development of new, more effective, drugs for treatment of viral infections.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM037188-24
Application #
8374419
Study Section
Xenobiotic and Nutrient Disposition and Action Study Section (XNDA)
Program Officer
Okita, Richard T
Project Start
1986-09-01
Project End
2014-05-30
Budget Start
2012-12-01
Budget End
2014-05-30
Support Year
24
Fiscal Year
2013
Total Cost
$479,517
Indirect Cost
$158,461
Name
University of Michigan Ann Arbor
Department
Pharmacology
Type
Schools of Pharmacy
DUNS #
073133571
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
Tsume, Yasuhiro; Mudie, Deanna M; Langguth, Peter et al. (2014) The Biopharmaceutics Classification System: subclasses for in vivo predictive dissolution (IPD) methodology and IVIVC. Eur J Pharm Sci 57:152-63
Walls, Zachary F; Gupta, Sheeba Varghese; Amidon, Gordon L et al. (2014) Synthesis and characterization of valyloxy methoxy luciferin for the detection of valacyclovirase and peptide transporter. Bioorg Med Chem Lett 24:4781-3
Tsume, Yasuhiro; Incecayir, Tuba; Song, Xueqin et al. (2014) The development of orally administrable gemcitabine prodrugs with D-enantiomer amino acids: enhanced membrane permeability and enzymatic stability. Eur J Pharm Biopharm 86:514-23
Incecayir, Tuba; Tsume, Yasuhiro; Amidon, Gordon L (2013) Comparison of the permeability of metoprolol and labetalol in rat, mouse, and Caco-2 cells: use as a reference standard for BCS classification. Mol Pharm 10:958-66
Gupta, Deepak; Varghese Gupta, Sheeba; Dahan, Arik et al. (2013) Increasing oral absorption of polar neuraminidase inhibitors: a prodrug transporter approach applied to oseltamivir analogue. Mol Pharm 10:512-22
Tsume, Yasuhiro; Amidon, Gordon L (2012) The feasibility of enzyme targeted activation for amino acid/dipeptide monoester prodrugs of floxuridine; cathepsin D as a potential targeted enzyme. Molecules 17:3672-89
Tsume, Yasuhiro; Hilfinger, John M; Amidon, Gordon L (2011) Potential of amino acid/dipeptide monoester prodrugs of floxuridine in facilitating enhanced delivery of active drug to interior sites of tumors: a two-tier monolayer in vitro study. Pharm Res 28:2575-88
Amidon, K S; Langguth, P; Lennernas, H et al. (2011) Bioequivalence of oral products and the biopharmaceutics classification system: science, regulation, and public policy. Clin Pharmacol Ther 90:467-70
Tsume, Yasuhiro; Provoda, Chester J; Amidon, Gordon L (2011) The achievement of mass balance by simultaneous quantification of floxuridine prodrug, floxuridine, 5-fluorouracil, 5-dihydrouracil, ?-fluoro-?-ureidopropionate, ?-fluoro-?-alanine using LC-MS. J Chromatogr B Analyt Technol Biomed Life Sci 879:915-20
Gupta, Sheeba Varghese; Gupta, Deepak; Sun, Jing et al. (2011) Enhancing the intestinal membrane permeability of zanamivir: a carrier mediated prodrug approach. Mol Pharm 8:2358-67

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