The yeast genes RNA2 through RNA11 are necessary for the processing of nuclear precursors in messenger RNA. Temperature sensitive recessive mutations in these genes result in the accumulation at the nonpermissive temperature of unspliced intron-containing precursors of mRNA. Evidence is accumulating that these RNA genes are directly involved in splicing. The RNA are in the yeast nucleus and temperature sensitive mutations in these RNA genes inactivate splicing extracts in vitro. A combined genetic, molecular biological, and biochemical approach will be taken to determine the function of the RNA2, RNA3, RNA4, and SRN2 genes in yeast mRNA splicing. Using the cloned RNA genes and antisera directed against the RNA gene products the following questions will be addressed: (1) Are the RNA gene products in the splicing complex - the spliceosome, or in snRNPs or HnRNPs? (2) What other molecules interact with the RNA gene products, and are they involved in mRNA splicing? (3) How do the RNA3 and RNA4, and SRN2 and RNA2 genes interact with each other? (4) Which steps in the assembly and function of the splicing complex require the function of RNA genes?

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM038782-05
Application #
3295458
Study Section
Biochemistry Study Section (BIO)
Project Start
1990-09-29
Project End
1995-08-31
Budget Start
1991-09-01
Budget End
1992-08-31
Support Year
5
Fiscal Year
1991
Total Cost
Indirect Cost
Name
Carnegie-Mellon University
Department
Type
Schools of Arts and Sciences
DUNS #
052184116
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213
Anthony, J G; Weidenhammer, E M; Woolford Jr, J L (1997) The yeast Prp3 protein is a U4/U6 snRNP protein necessary for integrity of the U4/U6 snRNP and the U4/U6.U5 tri-snRNP. RNA 3:1143-52
Weidenhammer, E M; Ruiz-Noriega, M; Woolford Jr, J L (1997) Prp31p promotes the association of the U4/U6 x U5 tri-snRNP with prespliceosomes to form spliceosomes in Saccharomyces cerevisiae. Mol Cell Biol 17:3580-8
Weidenhammer, E M; Singh, M; Ruiz-Noriega, M et al. (1996) The PRP31 gene encodes a novel protein required for pre-mRNA splicing in Saccharomyces cerevisiae. Nucleic Acids Res 24:1164-70
Maddock, J R; Roy, J; Woolford Jr, J L (1996) Six novel genes necessary for pre-mRNA splicing in Saccharomyces cerevisiae. Nucleic Acids Res 24:1037-44
Roy, J; Kim, K; Maddock, J R et al. (1995) The final stages of spliceosome maturation require Spp2p that can interact with the DEAH box protein Prp2p and promote step 1 of splicing. RNA 1:375-90
Roy, J; Zheng, B; Rymond, B C et al. (1995) Structurally related but functionally distinct yeast Sm D core small nuclear ribonucleoprotein particle proteins. Mol Cell Biol 15:445-55
Maddock, J R; Weidenhammer, E M; Adams, C C et al. (1994) Extragenic suppressors of Saccharomyces cerevisiae prp4 mutations identify a negative regulator of PRP genes. Genetics 136:833-47
Woolford Jr, J L (1989) Nuclear pre-mRNA splicing in yeast. Yeast 5:439-57