We seek to understand the molecules and forces that determine the orientation of cleavage planes in dividing frog eggs. This will generate fundamental knowledge relevant to division of all human cells, and may inform on the origin of certain types of birth defect. We will use eggs of the frog, Xenopus laevis, and extracts made from eggs that reconstitute the microtubule and actin based processes that organize early embryos in a cell-free system.
Our first aim i s to determine the mechanism by which radial arrays of microtubules, called asters, grow to fill the whole egg.
Our second aim i s to determine how signaling molecules that are recruited to the boundary between two microtubule asters signal to the plasma membrane to position cleavage furrows.
Our third aim i s to determine the mechanisms by which asters position in the egg, and by which centrioles positions inside asters.
We seek to understand the molecules and forces that control the geometry of dividing frog eggs. This will generate fundamental knowledge relevant to division of all human cells, and may inform on the origin of certain types of birth defect.
| Nguyen, P A; Field, C M; Mitchison, T J (2018) Prc1E and Kif4A control microtubule organization within and between large Xenopus egg asters. Mol Biol Cell 29:304-316 |
| Pineda, Javier J; Miller, Miles A; Song, Yuyu et al. (2018) Site occupancy calibration of taxane pharmacology in live cells and tissues. Proc Natl Acad Sci U S A 115:E11406-E11414 |
| Field, Christine M; Mitchison, Timothy J (2018) Assembly of Spindles and Asters in Xenopus Egg Extracts. Cold Spring Harb Protoc 2018:pdb.prot099796 |
| Liu, Ling; Su, Xiaoyang; Quinn 3rd, William J et al. (2018) Quantitative Analysis of NAD Synthesis-Breakdown Fluxes. Cell Metab 27:1067-1080.e5 |
| Presler, Marc; Van Itallie, Elizabeth; Klein, Allon M et al. (2017) Proteomics of phosphorylation and protein dynamics during fertilization and meiotic exit in the Xenopus egg. Proc Natl Acad Sci U S A 114:E10838-E10847 |
| Costigliola, Nancy; Ding, Liya; Burckhardt, Christoph J et al. (2017) Vimentin fibers orient traction stress. Proc Natl Acad Sci U S A 114:5195-5200 |
| Hanley, Mariah L; Yoo, Tae Yeon; Sonnett, Matthew et al. (2017) Chromosomal passenger complex hydrodynamics suggests chaperoning of the inactive state by nucleoplasmin/nucleophosmin. Mol Biol Cell 28:1444-1456 |
| Mitchison, Timothy J; Field, Christine M (2017) Spindle-to-Cortex Communication in Cleaving Frog Eggs. Cold Spring Harb Symp Quant Biol 82:165-171 |
| Guild, Joshua; Ginzberg, Miriam B; Hueschen, Christina L et al. (2017) Increased lateral microtubule contact at the cell cortex is sufficient to drive mammalian spindle elongation. Mol Biol Cell 28:1975-1983 |
| Boke, Elvan; Mitchison, Timothy J (2017) The balbiani body and the concept of physiological amyloids. Cell Cycle 16:153-154 |
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