Light provides energy for photosynthesis and also signals for plants to grow and develop. Arabidopsis contains several photoreceptors that monitor photons at different wavelengths. Amongst these, phytochrome A (phyA), which is activated by far-red (FR) light, plays a major role in seedling deetiolation. Our long-term objective is to understand the inter-relationship between the various signaling intermediates and how their interactions lead to transduction of phyA signals for downstream developmental responses. To this end, we will analyze mutants carrying multiple mutations with lesions in various combinations of phyA signaling factors to establish the hierarchical relationship between the two homologous proteins, FHY1/FHL, and three transcription activators, LAF1, HFR1 and HY5. Interaction domains will be mapped by in vitro pull- down assays. Factor association in vivo in response to darkness and FR will be determined by co-immunoprecipitation and the physiological impact on phyA responses will be assayed during seedling deetiolation. Control of phy A signaling factor abundances has emerged to be an essential regulatory mechanism and COP1, a negative regulator of phYA signaling, plays a major role. COP1 shows E3 ligase activity toward LAF1 and HFR1, which interact with one another in vitro. Using transgenic plants expressing both factors we will determine whether their association in vivo would lead to FR-hypersensitivity. Another COP1 target is the bZIP factor, HY5, which appears to mediate opposing effects on cell expansion in hypocotyls and cotyledons in response to FR light. We will investigate how HY5 differentially controls expression of the Membrane Steroid Binding Protein (MSBP) 1 in these two organs, which is known to be a negative regulator of cell expansion, Transgenic plants expressing the appropriate constructs will be used to determine whether regulation is at the transcriptional, post-transcriptional or post-translational level. Finally, we will explore whether miRNA and siRNAs are involved in the regulation of phyA signaling. We expect results from these experiments to uncover previously unknown aspects of phyA signaling and also provide additional information on general mechanisms of eukaryotic cell signaling.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM044640-20
Application #
8113388
Study Section
Membrane Biology and Protein Processing (MBPP)
Program Officer
Hagan, Ann A
Project Start
1991-04-01
Project End
2013-07-31
Budget Start
2011-08-01
Budget End
2013-07-31
Support Year
20
Fiscal Year
2011
Total Cost
$493,029
Indirect Cost
Name
Rockefeller University
Department
Biochemistry
Type
Other Domestic Higher Education
DUNS #
071037113
City
New York
State
NY
Country
United States
Zip Code
10065
Henriques, Rossana; Mas, Paloma (2013) Chromatin remodeling and alternative splicing: pre- and post-transcriptional regulation of the Arabidopsis circadian clock. Semin Cell Dev Biol 24:399-406
Jang, In-Cheol; Henriques, Rossana; Chua, Nam-Hai (2013) Three transcription factors, HFR1, LAF1 and HY5, regulate largely independent signaling pathways downstream of phytochrome A. Plant Cell Physiol 54:907-16
Hemmes, Hans; Henriques, Rossana; Jang, In-Cheol et al. (2012) Circadian clock regulates dynamic chromatin modifications associated with Arabidopsis CCA1/LHY and TOC1 transcriptional rhythms. Plant Cell Physiol 53:2016-29
Jakubiec, Anna; Yang, Seong Wook; Chua, Nam-Hai (2012) Arabidopsis DRB4 protein in antiviral defense against Turnip yellow mosaic virus infection. Plant J 69:14-25
Xu, Jun; Chua, Nam-Hai (2012) Dehydration stress activates Arabidopsis MPK6 to signal DCP1 phosphorylation. EMBO J 31:1975-84
Liu, Jun; Jung, Choonkyun; Xu, Jun et al. (2012) Genome-wide analysis uncovers regulation of long intergenic noncoding RNAs in Arabidopsis. Plant Cell 24:4333-45
Jang, In-Cheol; Chung, Pil Joong; Hemmes, Hans et al. (2011) Rapid and reversible light-mediated chromatin modifications of Arabidopsis phytochrome A locus. Plant Cell 23:459-70
Wang, Meimei; Soyano, Takashi; Machida, Satoru et al. (2011) Molecular insights into plant cell proliferation disturbance by Agrobacterium protein 6b. Genes Dev 25:64-76
Wang, Huan; Zhang, Xiuren; Liu, Jun et al. (2011) Deep sequencing of small RNAs specifically associated with Arabidopsis AGO1 and AGO4 uncovers new AGO functions. Plant J 67:292-304
Xu, Jun; Chua, Nam-Hai (2011) Processing bodies and plant development. Curr Opin Plant Biol 14:88-93

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