Abstract

The aim of this application is to characterize the molecular events involved in the assembly of gap junction channels. The proposal is divided into three aims. First, the investigators will characterize the mechanisms of post-translational control of connexin transport to the plasma membrane. Second, they will analyze the regulation of Cx43 hemichannel gating and the lateral migration of hemichannels. Third, they will study the mechanism(s) by which phosphorylation of Cx43 leads to an inhibition of Gj assembly and communication.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM046277-05A2
Application #
2404339
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1991-09-30
Project End
2001-06-30
Budget Start
1997-07-01
Budget End
1998-06-30
Support Year
5
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
University of Minnesota Twin Cities
Department
Genetics
Type
Schools of Arts and Sciences
DUNS #
168559177
City
Minneapolis
State
MN
Country
United States
Zip Code
55455

  Publications

Johnson, Ross G; Le, Hung C; Evenson, Kristen et al. (2016) Connexin Hemichannels: Methods for Dye Uptake and Leakage. J Membr Biol 249:713-741
Johnson, Ross G; Reynhout, James K; TenBroek, Erica M et al. (2012) Gap junction assembly: roles for the formation plaque and regulation by the C-terminus of connexin43. Mol Biol Cell 23:71-86
Essner, J J; Laing, J G; Beyer, E C et al. (1996) Expression of zebrafish connexin43.4 in the notochord and tail bud of wild-type and mutant no tail embryos. Dev Biol 177:449-62